Kobusiewicz W, Ogonowska-Kobusiewicz M, Michalak J, Zywicki W, Ledwozyw A
Clinic of Vascular Surgery, Medical Academy, Lublin, Poland.
Acta Vet Hung. 1995;43(2-3):311-20.
Ischaemic injury was produced in the dog heart by occluding the left anterior descending coronary artery just below the second diagonal branch for 3 h. Verapamil 5.0 mg/kg was injected intravenously 10 min before the occlusion. There was some decrease in ATP and creatine phosphate levels in the hearts of "no drug" animals as compared to sham-operated dogs. Verapamil significantly increase the level of these metabolites. The ATP/ADP ratio decreased from 8.5 +/- 0.60 in hearts from sham-operated dogs to 3.5 +/- 0.30 in the "no drug" group. Verapamil pretreatment resulted in values of the ATP/ADP ratio which were lower than, but not significantly different from, those observed in the control group. In ischaemic mitochondria the calcium content (Ca++) increased significantly as compared to the control and the Verapamil group. A significant increase in free fatty acid content in myocardial membrane preparations was also observed as compared to the sham-operated and the Verapamil group.
通过在第二对角支下方闭塞左前降支冠状动脉3小时，在犬心脏中制造缺血性损伤。在闭塞前10分钟静脉注射5.0mg/kg维拉帕米。与假手术犬相比，“无药物”动物心脏中的ATP和磷酸肌酸水平有所下降。维拉帕米显著提高了这些代谢物的水平。ATP/ADP比值从假手术犬心脏中的8.5±0.60降至“无药物”组中的3.5±0.30。维拉帕米预处理导致ATP/ADP比值低于对照组，但差异无统计学意义。与对照组和维拉帕米组相比，缺血线粒体中的钙含量（Ca++）显著增加。与假手术组和维拉帕米组相比，心肌膜制剂中的游离脂肪酸含量也显著增加。
