Clandinin M T, Zuberbuhler P, Brown N E, Kielo E S, Goh Y K
Department of Agriculture, University of Alberta, Edmonton, Canada.
Am J Clin Nutr. 1995 Dec;62(6):1268-75. doi: 10.1093/ajcn/62.6.1268.
The objective of this study was to quantitatively measure the essential fatty acid pools in lipid fractions of plasma lipoproteins and the alteration of these pool sizes after consumption of a physiologic intake of fish oil. Twenty-three cystic fibrosis (CF) patients and 21 normal subjects were supplemented with fish oil, providing 35 mg n-3 fatty acids/kg body wt for 4 wk. Blood was collected pre- and postsupplementation and was quantitatively analyzed to determine the fatty acid pool size in each lipid class of low-density lipoproteins (LDLs), high-density lipoproteins (HDLs), and very-low-density lipoproteins. Two 7-d food records were collected to determine total fat and fatty acid intakes. Intakes of protein, carbohydrate, and fat as a percentage of energy and the ratio of polyunsaturated to saturated fats was similar for the CF and control groups. Energy intake was greater for CF subjects. Smaller lipoprotein essential fatty acid pools were observed in CF patients than in control subjects. Healthy subjects had larger essential fatty acid pool sizes in cholesterol ester fractions of HDLs and LDLs than CF subjects. Cholesterol ester and phosphatidylcholine pools transported the majority of essential fatty acids in both CF and control subjects.
本研究的目的是定量测量血浆脂蛋白脂质组分中的必需脂肪酸池，以及在摄入生理剂量的鱼油后这些池大小的变化。23名囊性纤维化（CF）患者和21名正常受试者补充鱼油，按35 mg n-3脂肪酸/千克体重给予，为期4周。在补充前后采集血液，并进行定量分析，以确定低密度脂蛋白（LDL）、高密度脂蛋白（HDL）和极低密度脂蛋白各脂质类别的脂肪酸池大小。收集两份7天的食物记录，以确定总脂肪和脂肪酸摄入量。CF组和对照组的蛋白质、碳水化合物和脂肪摄入量占能量的百分比以及多不饱和脂肪与饱和脂肪的比例相似。CF受试者的能量摄入量更高。与对照组相比，CF患者的脂蛋白必需脂肪酸池较小。健康受试者HDL和LDL胆固醇酯组分中的必需脂肪酸池大小比CF受试者更大。在CF患者和对照组中，胆固醇酯和磷脂酰胆碱池运输了大部分必需脂肪酸。
