Ogle C K, Zuo L, Mao J X, Alexander J W, Fischer J E, Nussbaum M S
Department of Surgery, University of Cincinnati, Ohio, USA.
Arch Surg. 1995 Dec;130(12):1301-7; discussion 1307-8. doi: 10.1001/archsurg.1995.01430120055008.
To determine the effect of parenteral nutrition (PN) on the expression of message for inflammatory cytokines in the spleen and different segments of the intestine.
Randomized controlled trial.
Eleven adult male Sprague-Dawley rats weighing 250 to 300 g.
All rats underwent central venous cannulation and were randomized to two groups. Group 1 (n = 6) received saline solution infusion and chow ad libitum; group 2 (n = 5) received lipid-free PN with no oral feeding. After 7 days, the animals were killed and the spleens and segments of small and large intestine were removed.
The expression of message for tumor necrosis factor alpha (TNF-alpha), interleukin-6 (IL-6), and IL-1 in the spleen and intestine was determined using a semiquantitative reverse transcription polymerase reaction. Splenic macrophages were isolated and cultured for 24 hours with and without lipopolysaccharide. Production of TNF-alpha and IL-6 was determined by bioassay followed by enzyme-linked immunosorbent assay.
After 7 days of infusion, messenger RNA (mRNA) expression for TNF-alpha, IL-1, and IL-6 was increased in the jejunum (P < .05), and TNF-alpha mRNA and IL-6 mRNA expression was decreased in the spleen (P < .01) of PN-fed animals when compared with saline/chow controls. In addition, TNF-alpha mRNA expression was increased in the cecum (P < .05), IL-1 mRNA expression was increased in the ileum (P < .05), and IL-6 mRNA expression was increased in the cecum (P < .05) and Peyer's patches (P < .007) in the PN-fed animals. Production of TNF-alpha and IL-6 by splenic macrophages was decreased following PN infusion in both lipopolysaccharide-treated and untreated cultures (P < .05).
Infusion of lipid-free PN induces a differential mRNA expression for inflammatory cytokines in the spleen and intestine with an overall up-regulation of the expression of inflammatory cytokines in the intestine and a down-regulation in the spleen. These data provide evidence that the regulatory mechanisms for cytokine production are different in the intestine and the spleen. Further study is needed to elaborate the mechanism of this differential expression following lipid-free PN infusion.
确定肠外营养（PN）对脾脏及肠道不同节段中炎性细胞因子信使表达的影响。
随机对照试验。
11只体重250至300克的成年雄性斯普拉格-道利大鼠。
所有大鼠均进行中心静脉插管，并随机分为两组。第1组（n = 6）接受生理盐水输注并随意进食；第2组（n = 5）接受无脂PN且不进行经口喂养。7天后，处死动物，取出脾脏及小肠和大肠节段。
使用半定量逆转录聚合酶反应测定脾脏和肠道中肿瘤坏死因子α（TNF-α）、白细胞介素-6（IL-6）和IL-1信使的表达。分离脾巨噬细胞，在有和无脂多糖的情况下培养24小时。通过生物测定法随后进行酶联免疫吸附测定法测定TNF-α和IL-6的产生。
输注7天后，与生理盐水/随意进食对照组相比，接受PN喂养的动物空肠中TNF-α、IL-1和IL-6的信使核糖核酸（mRNA）表达增加（P <.05），脾脏中TNF-α mRNA和IL-6 mRNA表达降低（P <.01）。此外，接受PN喂养的动物盲肠中TNF-α mRNA表达增加（P <.05），回肠中IL-1 mRNA表达增加（P <.05），盲肠（P <.05）和派尔集合淋巴结（P <.007）中IL-6 mRNA表达增加。在脂多糖处理和未处理的培养物中，PN输注后脾巨噬细胞产生的TNF-α和IL-6均降低（P <.05）。
无脂PN输注可诱导脾脏和肠道中炎性细胞因子的mRNA表达差异，肠道中炎性细胞因子表达总体上调，而脾脏中则下调。这些数据证明细胞因子产生的调节机制在肠道和脾脏中有所不同。需要进一步研究以阐明无脂PN输注后这种差异表达的机制。
