Maccarrone M, Hilbers M P, Veldink G A, Vliegenthart J F, Finazzi Agrò A
Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Italy.
Biochim Biophys Acta. 1995 Oct 26;1259(1):1-3. doi: 10.1016/0005-2760(95)00153-4.
A number of plasmids were constructed containing chimeric genes consisting of fragments of antisense-oriented lentil lipoxygenase cDNA. The different constructs were tested for their ability to lower lipoxygenase activity in lentil protoplasts. Plasmids containing a full length lentil lipoxygenase cDNA proved to be the most effective, reducing the activity of the target enzyme to 70% of the control value. On the other hand, the full length lentil lipoxygenase cDNA in the sense orientation yielded a 20% increase of lipoxygenase activity.
构建了许多质粒，这些质粒包含由反义方向的小扁豆脂氧合酶cDNA片段组成的嵌合基因。测试了不同构建体降低小扁豆原生质体中脂氧合酶活性的能力。含有全长小扁豆脂氧合酶cDNA的质粒被证明是最有效的，将靶酶的活性降低到对照值的70%。另一方面，正义方向的全长小扁豆脂氧合酶cDNA使脂氧合酶活性提高了20%。
