从一个近乎全长的cDNA预测的豌豆（Pisum sativum）种子脂氧合酶的完整氨基酸序列。
The complete amino acid sequence of a pea (Pisum sativum) seed lipoxygenase predicted from a near full-length cDNA.
作者信息
Ealing P M, Casey R
机构信息
John Innes Institute, Norwich, U.K.
出版信息
Biochem J. 1988 Aug 1;253(3):915-8. doi: 10.1042/bj2530915.
A near full-length cDNA for a pea (Pisum sativum) seed lipoxygenase was isolated and sequenced. It has a protein coding sequence (2583 bp), 5' (59 bp) and 3' (191 bp) non-coding regions, and a poly(A) tail (20 bp). The predicted amino acid sequence indicates a polypeptide of Mr 97,628 that shows about 86% amino acid identity with a soya-bean lipoxygenase 3 protein sequence [Yenofsky, Fine & Liu (1988) Mol. Gen. Genet. 211, 215-222]. The cDNA directs the transcription of mRNA that can be translated to give an anti-lipoxygenase-precipitable polypeptide in vitro.
分离并测序了豌豆（Pisum sativum）种子脂氧合酶的一个近乎全长的cDNA。它具有一个蛋白质编码序列（2583 bp）、5'非编码区（59 bp）和3'非编码区（191 bp），以及一个聚腺苷酸尾（20 bp）。预测的氨基酸序列表明是一个Mr 97,628的多肽，与大豆脂氧合酶3蛋白序列显示约86%的氨基酸同一性[Yenofsky, Fine & Liu (1988) Mol. Gen. Genet. 211, 215 - 222]。该cDNA指导mRNA的转录，该mRNA在体外可被翻译产生一种能被抗脂氧合酶沉淀的多肽。
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