McDonagh P F, Wilson D S
Department of Surgery, College of Medicine, University of Arizona, Tucson, USA.
Artif Cells Blood Substit Immobil Biotechnol. 1995;23(3):439-47. doi: 10.3109/10731199509117960.
Due to their enhanced oxygen carrying characteristics, perfluorocarbon emulsions are valuable adjuncts to coronary revascularization procedures. However, the effects of perfluorocarbon emulsions on white blood cell function are unclear. The purpose of this study was to determine the effects of three perfluorocarbon emulsions on the adhesion characteristics of leukocytes. Heparinized blood from donor rats was mixed with either Phosphate Buffered Saline (PBS), PFB-1, PFB-2 (both non-complement activating perflubron emulsions, Alliance Pharmaceutical Corp.) or Fluosol (20% w/v PFC, Alpha Therapeutic) in a ratio of one part emulsion to six parts blood. The blood-emulsion mixtures were incubated for ten minutes at 37 degrees C, then passed down nylon wool leukocyte adhesion columns. Blood samples were counted for: Leukocytes, Granulocytes and Lymphocytes. % Cell Adherence was calculated as: (1-[WBC-out]/[WBC-in]). We found that, compared to PBS control, the granulocyte adherence to the nylon fiber was significantly increased for both Fluosol-20 and PFB-1 (P < 0.05). In contrast, PFB-2 (designed for reduced cell surface activity) caused no significant change in leukocyte adhesion. In fact, a modest decrease in granulocyte adhesion was observed with PFB-2. These findings suggest a direct effect of some, but not all, PFC emulsions on blood leukocyte function.
由于其增强的携氧特性，全氟碳乳液是冠状动脉血运重建手术中有价值的辅助剂。然而，全氟碳乳液对白细胞功能的影响尚不清楚。本研究的目的是确定三种全氟碳乳液对白细胞黏附特性的影响。将来自供体大鼠的肝素化血液与磷酸盐缓冲盐水（PBS）、PFB-1、PFB-2（均为非补体激活的全氟溴烷乳液，联合制药公司）或氟碳乳剂（20% w/v全氟碳，阿尔法治疗公司）按一份乳液与六份血液的比例混合。将血液-乳液混合物在37℃下孵育10分钟，然后通过尼龙棉白细胞黏附柱。对血样进行白细胞、粒细胞和淋巴细胞计数。细胞黏附率计算公式为：(1 - [流出白细胞数]/[流入白细胞数])。我们发现，与PBS对照相比，氟碳乳剂-20和PFB-1的粒细胞对尼龙纤维的黏附均显著增加（P < 0.05）。相比之下，PFB-2（设计用于降低细胞表面活性）对白细胞黏附没有显著影响。事实上，观察到PFB-2使粒细胞黏附略有下降。这些发现表明一些（但不是全部）全氟碳乳液对血液白细胞功能有直接影响。
