García-Encina G, Farrán R, Puig S, Serafini M T, Martínez L
Laboratorios Dr. Esteve, Barcelona, Spain.
J Chromatogr B Biomed Appl. 1995 Aug 4;670(1):103-10. doi: 10.1016/0378-4347(95)00138-9.
A totally automated liquid chromatographic assay method based on a Prospekt solid-phase extraction unit was developed for the analysis of lesopitron and its metabolite 5-hydroxylesopitron in human plasma. On-line solid-phase extraction of lesopitron, 5-hydroxylesopitron and its internal standard in human plasma was carried out using C2 cartridges. After washing, the test substances were eluted with mobile phase onto an ODS-2 Inertsil column and measured by fluorescence detection. The total time for one analysis was 25 min. The method developed was selective and linear in the concentration range from 1 to 40 ng/ml for both parent drug and metabolite. Recovery of lesopitron and 5-hydroxylesopitron were higher than 80% and the quantification limits were 1 ng/ml for both compounds. Coefficients of variation obtained for precision parameters were all below 14.5% and 13.9% for parent drug and metabolite, respectively. Good values of accuracy were also obtained.
建立了一种基于Prospekt固相萃取装置的全自动液相色谱分析方法，用于测定人血浆中的来索匹隆及其代谢物5-羟基来索匹隆。使用C2柱对人血浆中的来索匹隆、5-羟基来索匹隆及其内标进行在线固相萃取。洗涤后，将测试物质用流动相洗脱到ODS-2 Inertsil柱上，并用荧光检测法进行测定。一次分析的总时间为25分钟。所建立的方法对于母体药物和代谢物在1至40 ng/ml的浓度范围内具有选择性和线性。来索匹隆和5-羟基来索匹隆的回收率均高于80%，两种化合物的定量限均为1 ng/ml。母体药物和代谢物的精密度参数变异系数分别均低于14.5%和13.9%。还获得了良好的准确度值。
