Kintz P, Cirimele V, Tracqui A, Mangin P
Institut de Médecine Légale, Strasbourg, France.
J Chromatogr B Biomed Appl. 1995 Aug 4;670(1):162-6. doi: 10.1016/0378-4347(95)00160-k.
A procedure is presented for the simultaneous identification and quantification of amphetamine (AP), methamphetamine (MA), methylenedioxyamphetamine (MDA) and methylenedioxymethamphetamine (MDMA) in human hair. The method involves decontamination of hair with dichloromethane and warm water, heat-alkaline hydrolysis in the presence of deuterated internal standards, liquid-liquid extraction and gas chromatography-mass spectrometry after derivatization with pentafluoropropionic anhydride-pentafluoropropanol. The limit of detection for AP, MA and MDA was 0.05 ng/mg using a 50-mg hair sample; for MDMA it was 0.1 ng/mg. Coefficients of variation ranged from 7 to 18%. This assay has been successfully utilized in the evaluation of the deposition of the drugs in hair obtained from various parts of the anatomy of a stimulant abuser.
本文介绍了一种同时鉴定和定量人发中苯丙胺（AP）、甲基苯丙胺（MA）、亚甲基二氧基苯丙胺（MDA）和亚甲基二氧基甲基苯丙胺（MDMA）的方法。该方法包括用二氯甲烷和温水对头发进行去污处理，在氘代内标存在下进行热碱性水解，液-液萃取以及用五氟丙酸酐-五氟丙醇衍生化后进行气相色谱-质谱分析。使用50毫克头发样本时，AP、MA和MDA的检测限为0.05纳克/毫克；MDMA的检测限为0.1纳克/毫克。变异系数范围为7%至18%。该检测方法已成功用于评估从兴奋剂滥用者身体不同部位获取的头发中药物的沉积情况。
