Hemsley S W, Canfield P J, Husband A J
Department of Veterinary Pathology, University of Sydney, New South Wales, Australia.
Immunol Cell Biol. 1995 Aug;73(4):321-5. doi: 10.1038/icb.1995.49.
Antibodies raised against evolutionarily conserved intracytoplasmic peptide sequences of human T and B lymphocyte antigens and an antibody raised specifically against koala serum IgG were assessed for their capacity for immunoperoxidase staining of formalin fixed lymphoid tissues of koalas (Phascolarctos cinereus), common brushtail possums (Trichosurus vulpecula), common ringtail possums (Pseudocheirus peregrinus) and tammar wallabies (Macropus eugenii). Utilizing microwave pretreatment and a streptavidin biotin-horseradish peroxidase method for histochemical staining, polyclonal anti-human CD3 (for T cells), monoclonal anti-human CD5 (for T cells), monoclonal anti-human CD79b cy (for B cells) and polyclonal anti-koala IgG (for plasma cells) consistently stained lymphoid elements in a wide range of tissues from all four marsupial species. Monoclonal anti-human CD79a cy (for B cells) stained lymphoid elements in only brushtail possums. This is the first report of immunohistological staining of lymphocytes in Australian marsupials, thereby vastly increasing the scope for immunological studies in these species.
评估了针对人类T和B淋巴细胞抗原进化保守胞质内肽序列产生的抗体以及专门针对树袋熊血清IgG产生的抗体，用于对树袋熊（灰袋熊）、普通帚尾袋貂、普通环尾袋貂和塔马尔沙袋鼠福尔马林固定淋巴组织进行免疫过氧化物酶染色的能力。利用微波预处理和链霉亲和素生物素 - 辣根过氧化物酶方法进行组织化学染色，多克隆抗人CD3（针对T细胞）、单克隆抗人CD5（针对T细胞）、单克隆抗人CD79b cy（针对B细胞）和多克隆抗树袋熊IgG（针对浆细胞）始终能对来自所有四种有袋类物种的多种组织中的淋巴成分进行染色。单克隆抗人CD79a cy（针对B细胞）仅能对帚尾袋貂的淋巴成分进行染色。这是澳大利亚有袋类动物淋巴细胞免疫组织化学染色的首次报告，从而极大地扩展了这些物种免疫研究的范围。
