Singh N B, Srivastava K, Malaviya B, Kandpal H, Srivastava A, Gupta H P
Division of Microbiology and Endocrinology, Chattar Manzil Palace, Lucknow, India.
Immunol Cell Biol. 1995 Aug;73(4):372-6. doi: 10.1038/icb.1995.57.
Mycobacteria including Mycobacterium tuberculosis and Mycobacterium leprae possess multiple antigens some of which inhibit other anti-mycobacterial immune responses. Whole cell vaccines are not free from these suppressive molecules and may adversely affect the immunogenic response(s). Purified protein components having only immunogenic properties should prove to be superior vaccine(s). Mycobacterium habana, a candidate vaccine for mycobacterial infections has been dissected for analysing its antigenic myriad. A 65 kDa protein of this mycobacterium has been isolated and characterized for its protective and cell mediated immune responses. The protein was isolated in pure form using an isotachophoresis (SDS-PAGE filtration) technique and identified with low molecular weight markers along with mAb using the immunoblot technique. Mab IIH9 has identified a 65 kDa protein in M. habana. This protein has been found to be immunoprotective in mice against M. tuberculosis H37Rv infection. It generates high levels of DTH responses in mice against M. tuberculosis and M. leprae antigens and inhibits migration of sensitized cells under the antigenic influence of homologous and heterologous origin. Possibilities of developing this protein as a subunit vaccine are discussed in this report.
包括结核分枝杆菌和麻风分枝杆菌在内的分枝杆菌具有多种抗原，其中一些会抑制其他抗分枝杆菌免疫反应。全细胞疫苗无法避免这些抑制分子的存在，可能会对免疫反应产生不利影响。仅具有免疫原性的纯化蛋白成分应被证明是更优质的疫苗。哈瓦那分枝杆菌是一种用于分枝杆菌感染的候选疫苗，已对其进行剖析以分析其众多抗原。已分离出该分枝杆菌的一种65 kDa蛋白，并对其保护性和细胞介导的免疫反应进行了表征。使用等速电泳（SDS-PAGE过滤）技术以纯形式分离该蛋白，并使用免疫印迹技术与低分子量标记物以及单克隆抗体进行鉴定。单克隆抗体IIH9已鉴定出哈瓦那分枝杆菌中的一种65 kDa蛋白。已发现该蛋白在小鼠中对结核分枝杆菌H37Rv感染具有免疫保护作用。它在小鼠中针对结核分枝杆菌和麻风分枝杆菌抗原产生高水平的迟发型超敏反应，并在同源和异源抗原的影响下抑制致敏细胞的迁移。本报告讨论了将该蛋白开发为亚单位疫苗的可能性。
