Washburn L R, Voelker L L, Ehle L J, Hirsch S, Dutenhofer C, Olson K, Beck B
Department of Microbiology, School of Medicine, University of South Dakota, Vermillion 57069-2390, USA.
J Clin Microbiol. 1995 Sep;33(9):2271-9. doi: 10.1128/jcm.33.9.2271-2279.1995.
Twenty Mycoplasma arthritidis strains or isolates were compared by a combination of enzyme-linked immunosorbent assay by an antiserum adsorption technique, Western immunoblotting, and restriction analysis of chromosomal DNA. Antigenic markers that defined strains related to strains 158p10p9, PG6, and H606 were identified. In addition, restriction analysis allowed all 20 strains to be divided into six groups. Results of restriction analysis corresponded generally with antigenic similarities, although the former did not allow grouping with as fine a precision as the latter. However, intrastrain antigenic variability, which is common among many Mycoplasma species, including M. arthritidis, introduced a complicating factor into our attempts at antigenic analysis. While serologic and antigenic analyses remain useful, we recommend that they be used with caution and in combination with other techniques for identifying and characterizing new isolates and newly acquired strains. Combinations of these techniques have proven to be useful in our laboratory for quality control and for uncovering interesting relationships among strains subjected to animal passage and their less virulent antecedents and among strains originally classified as the same but obtained from different sources and maintained, sometimes for decades, in different laboratories.
通过抗血清吸附技术的酶联免疫吸附测定、Western免疫印迹和染色体DNA的限制性分析相结合的方法，对20株关节炎支原体菌株或分离株进行了比较。确定了与158p10p9、PG6和H606菌株相关的菌株的抗原标记。此外，限制性分析可将所有20株菌株分为六组。尽管前者的分组精度不如后者，但限制性分析的结果总体上与抗原相似性相符。然而，包括关节炎支原体在内的许多支原体物种中常见的菌株内抗原变异性，给我们的抗原分析尝试带来了一个复杂因素。虽然血清学和抗原分析仍然有用，但我们建议谨慎使用它们，并与其他技术结合使用，以鉴定和表征新的分离株和新获得的菌株。这些技术的组合已被证明在我们实验室中对于质量控制以及揭示经过动物传代的菌株与其毒性较低的前身之间以及最初分类相同但来自不同来源并在不同实验室（有时长达数十年）保存的菌株之间的有趣关系是有用的。
