AnaeroGen系统用于厌氧菌生长的评估。
Evaluation of AnaeroGen system for growth of anaerobic bacteria.
作者信息
Miller P H, Wiggs L S, Miller J M
机构信息
Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
出版信息
J Clin Microbiol. 1995 Sep;33(9):2388-91. doi: 10.1128/jcm.33.9.2388-2391.1995.
The Oxoid AnaeroGen system was compared with the BBL GasPak for the production of an anaerobic atmosphere and was evaluated for its ability to support the growth of 135 clinically significant anaerobic bacteria. An anaerobe chamber was used as the "gold standard" for supporting the growth of anaerobes. The AnaeroGen requires no catalyst, produces no hydrogen, requires no water, and reduces preparation time to a minimum. The water-activated BBL GasPak generates hydrogen. For 132 of the 135 strains tested, better initial growth at 48 h was noted for the jar methods than for the anaerobe chamber. At 72 h, 113 of the 135 strains showed equal growth, and at 7 days, only marginal differences in growth patterns were noted. The AnaeroGen never failed to reduce the anaerobic indicator, while the BBL GasPak occasionally failed to do so. The AnaeroGen performed at least as well as, and sometimes better than, the established methods. The AnaeroGen is a good alternative for use in anaerobic jars.
将Oxoid AnaeroGen系统与BBL GasPak用于产生厌氧环境的效果进行了比较，并评估了其支持135种具有临床意义的厌氧菌生长的能力。使用厌氧箱作为支持厌氧菌生长的“金标准”。AnaeroGen不需要催化剂，不产生氢气，不需要水，并将准备时间减至最短。水激活的BBL GasPak会产生氢气。在测试的135株菌株中，有132株在48小时时，采用培养罐法的初始生长情况比厌氧箱更好。在72小时时，135株菌株中有113株生长情况相同，在7天时，仅观察到生长模式上的微小差异。AnaeroGen从未未能使厌氧指示剂变色，而BBL GasPak偶尔会出现这种情况。AnaeroGen的表现至少与既定方法相当，有时甚至更好。AnaeroGen是厌氧培养罐使用的一个良好替代品。
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