一种用于人细小病毒B19感染常规诊断的简单且灵敏的DNA杂交检测方法。

A simple and sensitive DNA hybridization assay used for the routine diagnosis of human parvovirus B19 infection.

作者信息

Hicks K E, Beard S, Cohen B J, Clewley J P

机构信息

Virus Reference Division, Central Public Health Laboratory, London, United Kingdom.

出版信息

J Clin Microbiol. 1995 Sep;33(9):2473-5. doi: 10.1128/jcm.33.9.2473-2475.1995.

DOI:10.1128/jcm.33.9.2473-2475.1995

PMID:7494051

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228444/

Abstract

A dot blot hybridization assay for parvovirus B19 diagnosis was developed by using a PCR-generated probe, digoxigenin labelling, and chemiluminescence detection. Different labelling techniques and hybridization solutions were evaluated. From this analysis a protocol was devised for routine diagnostic use. The protocol enabled 1 pg of B19 DNA to be detected. The results of applying this method to 8,369 diagnostic samples collected during 1994 and 1995 are given.

摘要

利用聚合酶链反应（PCR）生成的探针、地高辛标记和化学发光检测技术，开发了一种用于诊断细小病毒B19的斑点杂交试验。对不同的标记技术和杂交溶液进行了评估。通过该分析设计了一种用于常规诊断的方案。该方案能够检测到1 pg的B19 DNA。给出了将该方法应用于1994年和1995年收集的8369份诊断样本的结果。