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Rapid detection of PCR products of hepatitis C virus (HCV) with a non-radioactive oligoprobe.

作者信息

Canepari P, Di Stefano C F, Lleò M M

机构信息

Istituto di Microbiologia dell'Università di Verona, Italy.

出版信息

New Microbiol. 1994 Jan;17(1):9-14.

PMID:8127233
Abstract

Dot-blot for rapid detection of PCR amplification products of hepatitis C virus (HCV) using a digoxigenin (DIG)-labelled oligoprobe was developed and its sensitivity compared with Southern blot hybridization. The specificity and sensitivity of the DIG-labelled probe were identical to those of the 32P-labelled when the DIG-labelled nucleic acids were detected by enzyme-catalyzed chemiluminescent reaction. The lack of radioactivity makes this procedure suitable for routine use in diagnostic laboratories.

摘要

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Rapid detection of PCR products of hepatitis C virus (HCV) with a non-radioactive oligoprobe.
New Microbiol. 1994 Jan;17(1):9-14.
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