利用合成肽制备的单克隆抗体对耐甲氧西林葡萄球菌青霉素结合蛋白2'进行免疫检测。
Immunological detection of penicillin-binding protein 2' of methicillin-resistant staphylococci by using monoclonal antibodies prepared from synthetic peptides.
作者信息
Saito M, Sekiguchi K, Yajima R, Hina M, Doss R C, Kanno H
机构信息
Research and Development Center, Dainabot Co., Ltd., Chiba, Japan.
出版信息
J Clin Microbiol. 1995 Sep;33(9):2498-500. doi: 10.1128/jcm.33.9.2498-2500.1995.
Abstract
Two synthetic peptides 31 and 32 amino acids in length were prepared as deduced from a known amino acid sequence of penicillin-binding protein 2' (PBP2') of methicillin-resistant Staphylococcus aureus. Two monoclonal antibodies were generated from fused cells of myeloma cells and splenic cells of mice immunized with the synthetic peptides. Western blot (immunoblot) analysis demonstrated specific binding of the antibodies to PBP2' of a methicillin-resistant S. aureus strain. An immunoradiometric assay was developed by using these antibodies for simple detection of PBP2'.
摘要
根据耐甲氧西林金黄色葡萄球菌青霉素结合蛋白2'（PBP2'）的已知氨基酸序列推导，制备了两种长度分别为31个和32个氨基酸的合成肽。用这些合成肽免疫小鼠，从小鼠的骨髓瘤细胞和脾细胞的融合细胞中产生了两种单克隆抗体。蛋白质免疫印迹（免疫印迹）分析表明，这些抗体与耐甲氧西林金黄色葡萄球菌菌株的PBP2'具有特异性结合。利用这些抗体开发了一种免疫放射分析方法，用于简单检测PBP2'。
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