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1
Immunological detection of penicillin-binding protein 2' of methicillin-resistant staphylococci by using monoclonal antibodies prepared from synthetic peptides.利用合成肽制备的单克隆抗体对耐甲氧西林葡萄球菌青霉素结合蛋白2'进行免疫检测。
J Clin Microbiol. 1995 Sep;33(9):2498-500. doi: 10.1128/jcm.33.9.2498-2500.1995.
2
Detection of methicillin-resistant Staphylococcus aureus (MRSA) with antibodies against synthetic peptides derived from penicillin-binding protein 2'.用针对源自青霉素结合蛋白2'的合成肽的抗体检测耐甲氧西林金黄色葡萄球菌（MRSA）
Microbiol Immunol. 1995;39(8):545-50. doi: 10.1111/j.1348-0421.1995.tb02240.x.
3
Degradation of penicillin-binding protein 2' in methicillin-resistant Staphylococcus aureus.耐甲氧西林金黄色葡萄球菌中青霉素结合蛋白2'的降解
Chemotherapy. 1995 May-Jun;41(3):172-7. doi: 10.1159/000239340.
4
Immunological detection of penicillin-binding protein 2' in clinical isolates of methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis.耐甲氧西林金黄色葡萄球菌和表皮葡萄球菌临床分离株中青霉素结合蛋白2'的免疫学检测
FEMS Microbiol Lett. 1989 Jan 1;48(1):97-103. doi: 10.1016/0378-1097(89)90154-7.
5
Characterisation of a monoclonal antibody and its use in the immunoaffinity purification of penicillin-binding protein 2' of methicillin-resistant Staphylococcus aureus.一种单克隆抗体的特性及其在耐甲氧西林金黄色葡萄球菌青霉素结合蛋白2'免疫亲和纯化中的应用。
FEMS Microbiol Lett. 1989 Nov;53(1-2):143-7. doi: 10.1016/0378-1097(89)90381-9.
6
Detection of penicillin-binding protein 2a by rapid slide latex agglutination test in coagulase-negative staphylococci.用快速玻片乳胶凝集试验检测凝固酶阴性葡萄球菌中的青霉素结合蛋白2a
J Clin Microbiol. 2001 Jan;39(1):412. doi: 10.1128/JCM.39.1.412.2001.
7
Nucleotide sequence of the structural gene for the penicillin-binding protein 2 of Staphylococcus aureus and the presence of a homologous gene in other staphylococci.金黄色葡萄球菌青霉素结合蛋白2结构基因的核苷酸序列以及其他葡萄球菌中同源基因的存在
FEMS Microbiol Lett. 1994 Apr 1;117(2):131-6. doi: 10.1111/j.1574-6968.1994.tb06754.x.
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Fosfomycin enhances the expression of penicillin-binding protein 2 in methicillin-sensitive and methicillin-resistant Staphylococcus aureus strains.
FEMS Microbiol Lett. 1992 Oct 15;76(3):221-6. doi: 10.1016/0378-1097(92)90340-t.
9
Role of an altered penicillin-binding protein in methicillin- and cephem-resistant Staphylococcus aureus.一种改变的青霉素结合蛋白在耐甲氧西林和头孢菌素金黄色葡萄球菌中的作用。
Antimicrob Agents Chemother. 1985 Sep;28(3):397-403. doi: 10.1128/AAC.28.3.397.
10
Characterization of an isogenic set of methicillin-resistant and susceptible mutants of Staphylococcus aureus.金黄色葡萄球菌耐甲氧西林和敏感突变体同基因集的表征
Eur J Clin Microbiol. 1986 Dec;5(6):697-701. doi: 10.1007/BF02013308.

引用本文的文献

1
Rapid slide latex agglutination test for detection of methicillin resistance in Staphylococcus aureus.用于检测金黄色葡萄球菌耐甲氧西林的快速玻片乳胶凝集试验。
J Clin Microbiol. 1999 Sep;37(9):2789-92. doi: 10.1128/JCM.37.9.2789-2792.1999.
2
Suppression of methicillin resistance in a mecA-containing pre-methicillin-resistant Staphylococcus aureus strain is caused by the mecI-mediated repression of PBP 2' production.含mecA的耐甲氧西林金黄色葡萄球菌菌株中甲氧西林耐药性的抑制是由mecI介导的对PBP 2'产生的抑制作用所导致的。
Antimicrob Agents Chemother. 1996 Dec;40(12):2680-5. doi: 10.1128/AAC.40.12.2680.
3
Waterborne protozoan pathogens.水传播的原生动物病原体。
Clin Microbiol Rev. 1997 Jan;10(1):67-85. doi: 10.1128/CMR.10.1.67.

本文引用的文献

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A simple method for displaying the hydropathic character of a protein.一种展示蛋白质亲水性特征的简单方法。
J Mol Biol. 1982 May 5;157(1):105-32. doi: 10.1016/0022-2836(82)90515-0.
2
The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.通过与含¹²⁵I的酰化剂结合将蛋白质标记至高比放射性。
Biochem J. 1973 Jul;133(3):529-39. doi: 10.1042/bj1330529.
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Role of an altered penicillin-binding protein in methicillin- and cephem-resistant Staphylococcus aureus.一种改变的青霉素结合蛋白在耐甲氧西林和头孢菌素金黄色葡萄球菌中的作用。
Antimicrob Agents Chemother. 1985 Sep;28(3):397-403. doi: 10.1128/AAC.28.3.397.
4
Occurrence of a beta-lactam-inducible penicillin-binding protein in methicillin-resistant staphylococci.耐甲氧西林葡萄球菌中β-内酰胺诱导型青霉素结合蛋白的出现。
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Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus by gene fusion.通过基因融合在耐甲氧西林金黄色葡萄球菌中诱导型青霉素靶蛋白的进化
FEBS Lett. 1987 Aug 31;221(1):167-71. doi: 10.1016/0014-5793(87)80373-3.
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Cloning and expression of methicillin resistance from Staphylococcus epidermidis in Staphylococcus carnosus.表皮葡萄球菌的耐甲氧西林特性在肉葡萄球菌中的克隆与表达。
Antimicrob Agents Chemother. 1988 Oct;32(10):1494-9. doi: 10.1128/AAC.32.10.1494.
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The international spread of methicillin-resistant Staphylococcus aureus.耐甲氧西林金黄色葡萄球菌的国际传播
J Hosp Infect. 1987 Jan;9(1):60-71. doi: 10.1016/0195-6701(87)90097-1.
8
Presence of an additional penicillin-binding protein in methicillin-resistant Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus hominis, and Staphylococcus simulans with a low affinity for methicillin, cephalothin, and cefamandole.耐甲氧西林表皮葡萄球菌、溶血葡萄球菌、人葡萄球菌和模仿葡萄球菌中存在一种对甲氧西林、头孢噻吩和头孢孟多亲和力低的额外青霉素结合蛋白。
Antimicrob Agents Chemother. 1990 Sep;34(9):1691-4. doi: 10.1128/AAC.34.9.1691.
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Sequence comparison of mecA genes isolated from methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis.从耐甲氧西林金黄色葡萄球菌和表皮葡萄球菌中分离出的mecA基因的序列比较。
Gene. 1990 Sep 28;94(1):137-8. doi: 10.1016/0378-1119(90)90481-6.
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Nonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus.用于检测金黄色葡萄球菌中耐甲氧西林基因的非放射性DNA探针。
Antimicrob Agents Chemother. 1991 Mar;35(3):575-8. doi: 10.1128/AAC.35.3.575.

利用合成肽制备的单克隆抗体对耐甲氧西林葡萄球菌青霉素结合蛋白2'进行免疫检测。

Immunological detection of penicillin-binding protein 2' of methicillin-resistant staphylococci by using monoclonal antibodies prepared from synthetic peptides.

作者信息

Saito M, Sekiguchi K, Yajima R, Hina M, Doss R C, Kanno H

机构信息

Research and Development Center, Dainabot Co., Ltd., Chiba, Japan.

出版信息

J Clin Microbiol. 1995 Sep;33(9):2498-500. doi: 10.1128/jcm.33.9.2498-2500.1995.

DOI:10.1128/jcm.33.9.2498-2500.1995
PMID:7494059
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228456/
Abstract

Two synthetic peptides 31 and 32 amino acids in length were prepared as deduced from a known amino acid sequence of penicillin-binding protein 2' (PBP2') of methicillin-resistant Staphylococcus aureus. Two monoclonal antibodies were generated from fused cells of myeloma cells and splenic cells of mice immunized with the synthetic peptides. Western blot (immunoblot) analysis demonstrated specific binding of the antibodies to PBP2' of a methicillin-resistant S. aureus strain. An immunoradiometric assay was developed by using these antibodies for simple detection of PBP2'.

摘要

根据耐甲氧西林金黄色葡萄球菌青霉素结合蛋白2'（PBP2'）的已知氨基酸序列推导，制备了两种长度分别为31个和32个氨基酸的合成肽。用这些合成肽免疫小鼠，从小鼠的骨髓瘤细胞和脾细胞的融合细胞中产生了两种单克隆抗体。蛋白质免疫印迹（免疫印迹）分析表明，这些抗体与耐甲氧西林金黄色葡萄球菌菌株的PBP2'具有特异性结合。利用这些抗体开发了一种免疫放射分析方法，用于简单检测PBP2'。