用于检测金黄色葡萄球菌中耐甲氧西林基因的非放射性DNA探针。
Nonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus.
作者信息
Ligozzi M, Rossolini G M, Tonin E A, Fontana R
机构信息
Institute of Microbiology, University of Verona, Italy.
出版信息
Antimicrob Agents Chemother. 1991 Mar;35(3):575-8. doi: 10.1128/AAC.35.3.575.
Abstract
A DNA probe was developed by inserting, in the SmaI site of pBluescript sK, a 528-bp fragment of the gene responsible for intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus (mec determinant). The mec probe provided a useful tool for the rapid identification of the intrinsic resistance trait and for establishing guidelines for testing the in vitro susceptibility of S. aureus to beta-lactams.
摘要
通过将金黄色葡萄球菌中负责对β-内酰胺抗生素固有耐药性的基因（mec决定簇）的一个528碱基对片段插入到pBluescript sK的SmaI位点，构建了一个DNA探针。该mec探针为快速鉴定固有耐药性状以及制定检测金黄色葡萄球菌对β-内酰胺类药物体外敏感性的指南提供了一个有用的工具。
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