Seki K, Sakurada J, Murai M, Usui A, Seong H K, Jitsukawa H, Masuda S
Department of Bacteriology, Jikei University School of Medicine, Tokyo, Japan.
Microbiol Immunol. 1995;39(8):615-7. doi: 10.1111/j.1348-0421.1995.tb02250.x.
A supportive method for clonal identification of Staphylococcus aureus strains was devised. Culture supernatant obtained by cellophane surface culture was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) without performing any concentration procedure prior to electrophoresis. The combined use of cellophane surface culture and SDS-PAGE was convenient for determining whether the strains belonged to the same clone or not when conducted in conjunction with other tests for bacteriological characterization.
设计了一种用于金黄色葡萄球菌菌株克隆鉴定的支持性方法。通过玻璃纸表面培养获得的培养上清液，在电泳前不进行任何浓缩步骤，直接进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）。当与其他细菌学特征测试结合使用时，玻璃纸表面培养和SDS-PAGE的联合使用便于确定菌株是否属于同一克隆。
