通过十二烷基硫酸钠-聚丙烯酰胺凝胶上释放蛋白的蛋白条带模式对金黄色葡萄球菌进行克隆鉴定的辅助方法。

Auxiliary method for clonal identification of Staphylococcus aureus by protein band pattern of released proteins on SDS-polyacrylamide gel.

作者信息

Seki K, Sakurada J, Murai M, Usui A, Seong H K, Jitsukawa H, Masuda S

机构信息

Department of Bacteriology, Jikei University School of Medicine, Tokyo, Japan.

出版信息

Microbiol Immunol. 1995;39(8):615-7. doi: 10.1111/j.1348-0421.1995.tb02250.x.

DOI:10.1111/j.1348-0421.1995.tb02250.x

PMID:7494501

Abstract

A supportive method for clonal identification of Staphylococcus aureus strains was devised. Culture supernatant obtained by cellophane surface culture was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) without performing any concentration procedure prior to electrophoresis. The combined use of cellophane surface culture and SDS-PAGE was convenient for determining whether the strains belonged to the same clone or not when conducted in conjunction with other tests for bacteriological characterization.

摘要

设计了一种用于金黄色葡萄球菌菌株克隆鉴定的支持性方法。通过玻璃纸表面培养获得的培养上清液，在电泳前不进行任何浓缩步骤，直接进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）。当与其他细菌学特征测试结合使用时，玻璃纸表面培养和SDS-PAGE的联合使用便于确定菌株是否属于同一克隆。