Stephenson J R, Crook S J, Tabaqchali S
Br Med J (Clin Res Ed). 1986 Sep 6;293(6547):581-3. doi: 10.1136/bmj.293.6547.581.
Sodium dodecyl sulphate polyacrylamide gel electrophoresis of sulphur-35 methionine labelled cellular proteins of Staphylococcus aureus resistant to methicillin was used as a typing method during an outbreak on a cardiothoracic ward. This showed that the outbreak strain was indistinguishable from the epidemic strain of methicillin resistant S aureus prevalent in London. In contrast, 44 epidemiologically separate strains of S aureus gave individually distinct radiolabelled protein profiles. This method permitted rapid confirmation that an epidemic strain was responsible and indicated the need for urgent control measures.
在心胸外科病房爆发疫情期间，采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳法对耐甲氧西林金黄色葡萄球菌的硫-35蛋氨酸标记细胞蛋白进行分型。结果显示，爆发菌株与伦敦流行的耐甲氧西林金黄色葡萄球菌流行菌株无法区分。相比之下，44株在流行病学上相互独立的金黄色葡萄球菌菌株呈现出各自独特的放射性标记蛋白图谱。该方法能够快速确认流行菌株，并表明需要采取紧急控制措施。
