Hanihara T, Amano N, Takahashi T, Nagatomo H, Yagashita S
Department of Neurology, Kanagawa Rehabilitation Centre, Japan.
Neuropathol Appl Neurobiol. 1995 Aug;21(4):319-26. doi: 10.1111/j.1365-2990.1995.tb01066.x.
Recent studies have described silver- and tau-positive glia and threads in the degenerating lesions of progressive supranuclear palsy. In this study, Gallyas-Braak silver impregnation and several immunohistochemical techniques were employed to examine the distribution of tangles, abnormal glia and threads in the cerebral cortex of nine cases of progressive supranuclear palsy. In addition to neurofibrillary tangles, argentophilic glia and threads were impregnated exclusively by Gallyas-Braak technique. This technique demonstrated two types of glia profiles: tightly coiled intra-cytoplasmic profiles surrounding nuclei (coiled profiles) and thorn-like profiles with radial ramifications (thorn-like profiles). Thorn-like profiles are possibly in astrocytes and were detected in the cerebral cortex, while coiled profiles are possibly in oligodendroglia and were detected both in the cerebral cortex and subcortical white matter. Topographically, many neurofibrillary tangles were constantly seen in the frontal cortex and in the pre-central gyrus. Numerous neurofibrillary tangles were detected in the entorhinal cortex of the two brains. Argentophilic glia and threads were also frequent both in the frontal cortex and the precentral gyrus; however, they were more frequent in the pre-central gyrus that in the frontal cortex in four of the eight cases examined. In two brains, argentophilic threads were distributed widely in the cerebral cortex and white matter except for the temporal cortex. In immunohistochemical studies, argentophilic glia and threads were mostly positive for Tau 2, and a small number of them were weakly positive for ubiquitin and paired helical filament protein. The immunoproperties of these abnormal glia and threads seemed to be virtually identical to those of neurofibrillary tangles.(ABSTRACT TRUNCATED AT 250 WORDS)
近期研究描述了进行性核上性麻痹变性病灶中银和tau蛋白阳性的胶质细胞及细丝。在本研究中，采用加利亚斯-布拉克银浸染法及多种免疫组化技术，检测9例进行性核上性麻痹患者大脑皮质中缠结、异常胶质细胞及细丝的分布情况。除神经原纤维缠结外，加利亚斯-布拉克技术仅能浸染嗜银性胶质细胞及细丝。该技术显示出两种胶质细胞形态：围绕细胞核紧密盘绕的胞质内形态（盘绕形态）和具有放射状分支的刺状形态（刺状形态）。刺状形态可能存在于星形胶质细胞中，在大脑皮质中可检测到，而盘绕形态可能存在于少突胶质细胞中，在大脑皮质和皮质下白质中均能检测到。在地形学上，额叶皮质和中央前回经常可见许多神经原纤维缠结。在两个大脑的内嗅皮质中检测到大量神经原纤维缠结。嗜银性胶质细胞及细丝在额叶皮质和中央前回也很常见；然而，在8例受检病例中的4例中，它们在中央前回比在额叶皮质更常见。在两个大脑中，嗜银细丝广泛分布于大脑皮质和白质，但颞叶皮质除外。在免疫组化研究中，嗜银性胶质细胞及细丝大多对Tau 2呈阳性，少数对泛素和双螺旋丝蛋白呈弱阳性。这些异常胶质细胞及细丝的免疫特性似乎与神经原纤维缠结的免疫特性几乎相同。（摘要截选至250词）
