Perez H A, Wide A, Bracho C, de la Rosa M
Instituto Venezolano de Investigaciones Científicas, IVIC, Centro de Microbiología y Biología Celular, Caracas.
Parasite Immunol. 1995 Jun;17(6):305-12. doi: 10.1111/j.1365-3024.1995.tb00896.x.
A monoclonal antibody (MoAb) 2C6111 specific for Plasmodium vivax erythrocytic stages was shown to detect parasitized erythrocytes in blood samples collected in the field. This MoAb binds to the mature trophozoite, schizont and gametes of P. vivax and upon examination of 43 wild isolates no evidence of polymorphism was found. To search for P. vivax parasites in human blood a MoAb immunofluorescent test (MoAb-IFT) was developed. The assay is based on the ability of fluorescein isothiocyanate labelled MoAb 2C6111 to combine with parasitized erythrocytes on thin blood smears. A preliminary field trial was carried out in Venezuela to determine the usefulness of MoAb-IFT for the specific diagnosis of P. vivax malaria. Blood samples collected from malarious and non-malarious individuals were examined both by standard microscopy of Giemsa stained thick blood smears (G-TS) and MoAb-IFT. The latter was specific and gave a 100% correlation with G-TS. Sensitivity was close to that usually achieved with Giemsa stained blood films.
一种针对间日疟原虫红细胞内期的单克隆抗体（MoAb）2C6111被证明可检测在现场采集的血样中的被寄生红细胞。该单克隆抗体与间日疟原虫的成熟滋养体、裂殖体和配子结合，在对43株野外分离株进行检测时未发现多态性证据。为了在人血中寻找间日疟原虫寄生虫，开发了一种单克隆抗体免疫荧光试验（MoAb-IFT）。该检测方法基于异硫氰酸荧光素标记的单克隆抗体2C6111与薄血涂片上被寄生红细胞结合的能力。在委内瑞拉进行了一项初步现场试验，以确定MoAb-IFT对间日疟原虫疟疾特异性诊断的实用性。从疟疾患者和非疟疾患者采集的血样通过吉姆萨染色厚血涂片（G-TS）的标准显微镜检查和MoAb-IFT进行检测。后者具有特异性，与G-TS的相关性为100%。灵敏度与吉姆萨染色血片通常达到的灵敏度相近。
