用于检测血液期疟疾抗原的双位点全物种单克隆抗体酶联免疫吸附测定

Two-site pan-species monoclonal antibody ELISA for detection of blood stage malaria antigen.

作者信息

Gao Q, Tharavanij S, Khusmith S, Wang L Q, Looareesuwan S, Zhang W G

机构信息

Jiangsu Institute of Parasitic Diseases, Wuxi, People's Republic of China.

出版信息

Southeast Asian J Trop Med Public Health. 1992 Dec;23(4):740-4.

PMID:1298083

Abstract

A two-site pan-species monoclonal antibody sandwich ELISA (MAb-MAb ELISA) was developed to detect both Plasmodium vivax and P. falciparum antigens in whole blood impregnated on filter paper. In this assay, the plates were coated with pan-species MAb 3F9 and another pan-species MAb M26-32 conjugated with alkaline phosphatase was used for detection of bound antigen. The sensitivity of this assay was 5, 10 and 10 parasites per 10(6) erythrocytes for cultured P. falciparum, patient-derived P. vivax and P. falciparum, respectively. The coincidence rates for this assay were 93% (92/99) with healthy individuals and 93% (42/45) with microscopically confirmed vivax malaria cases. After two weeks treatment, 77.7% (14/18) of vivax malaria were still positive by this assay but with diminished level of reactivities [corrected].

摘要

开发了一种双位点全物种单克隆抗体夹心酶联免疫吸附测定法（MAb-MAb ELISA），用于检测浸渍在滤纸上的全血中的间日疟原虫和恶性疟原虫抗原。在该测定中，酶标板用全物种单克隆抗体3F9包被，并用另一种与碱性磷酸酶偶联的全物种单克隆抗体M26-32检测结合的抗原。该测定法对培养的恶性疟原虫、患者来源的间日疟原虫和恶性疟原虫的敏感性分别为每10⁶个红细胞中有5个、10个和10个疟原虫。该测定法与健康个体的符合率为93%（92/99），与显微镜确诊的间日疟病例的符合率为93%（42/45）。经过两周治疗后，77.7%（14/18）的间日疟病例通过该测定法仍呈阳性，但反应性水平降低[校正后]。