Theise H, Noll F, Bielka H
Acta Biol Med Ger. 1978;37(9):1353-62.
By combination of ion exchange chromatography, gel filtration, preparative polyacrylamide gel electrophoresis and perchloric acid fractionation 21 proteins of the small ribosomal subunit of rat liver were isolated with a purity of more than 95%. It could be demonstrated by immunological studies that no extensive structural homologies exist between these proteins.
通过离子交换色谱、凝胶过滤、制备性聚丙烯酰胺凝胶电泳和高氯酸分级分离相结合的方法,分离出了大鼠肝脏小核糖体亚基的21种蛋白质,其纯度超过95%。免疫学研究表明,这些蛋白质之间不存在广泛的结构同源性。