Lutsch G, Noll F, Theise H, Enzmann G, Bielka H
Mol Gen Genet. 1979 Oct 3;176(2):281-91. doi: 10.1007/BF00273223.
Ribosomal proteins S1, S2, S16 and S23 were localized on the surface of the small subunit (40S) of rat liver ribosomes by immune electron microscopy. Antibodies against the single proteins were raised in rabbits and chicken and purified by affinity chromatography. 40S-IgG-40S complexes were obtained by incubation of 40S subunits with non-cross-reacting antibodies specific for each of the four proteins and subsequent sucrose density gradient centrifugation. The location of the proteins was determined by means of antibody binding sites visualized in negative contrast in the electron microscope. The four investigated proteins are mainly located in the head region of the small subunit. Exposed antigenic determinants of proteins S1 and S2 were found to be located at different sites of the small subunit whereas proteins S16 and S23 were mapped in a limited region only.
通过免疫电子显微镜技术，核糖体蛋白S1、S2、S16和S23定位于大鼠肝脏核糖体小亚基（40S）的表面。针对这些单一蛋白的抗体在兔和鸡体内产生，并通过亲和层析进行纯化。通过将40S亚基与针对这四种蛋白中每一种的非交叉反应抗体孵育，随后进行蔗糖密度梯度离心，获得了40S-IgG-40S复合物。通过在电子显微镜下负染观察到的抗体结合位点来确定蛋白的位置。所研究的这四种蛋白主要位于小亚基的头部区域。发现蛋白S1和S2暴露的抗原决定簇位于小亚基的不同位点，而蛋白S16和S23仅定位在一个有限的区域。
