Creamer D, Allen M, Sousa A, Poston R, Barker J
St. John's Institute of Dermatology, UMDS, London, United Kingdom.
Am J Pathol. 1995 Dec;147(6):1661-7.
Considerable evidence indicates that microvascular changes observed in psoriasis are a result of vascular proliferation. A critical step in the sequence of events leading to neovascularization involves interactions between endothelial cells and extracellular matrix proteins mediated in part by the integrin family of adhesion molecules. A number of endothelial integrins have been shown to participate in neovascularization, including members of the beta 1, beta 3, and beta 4 subfamilies. To investigate the role of these integrins in psoriasis, specimens of lesional and nonlesional skin were taken from 10 patients with active, untreated plaque disease. Vascular endothelium was labeled with monoclonal antibodies specific for alpha 2, alpha 5, alpha 6, beta 1, av beta 3, and beta 4 integrins. The use of image analysis permitted quantification of immunoperoxidase staining and comparison of endothelial labeling in lesional and nonlesional skin. There was a significant increase in endothelial staining of av beta 3 integrin in lesional compared with nonlesional skin, both in superficial and deep vasculature. In contrast, there was a significant decrease in endothelial beta 4 staining in lesional compared with nonlesional superficial dermal vessels, alpha 2, alpha 5, alpha 6, and beta 1 staining showed no significant difference between the two groups. These results demonstrate an important role of av beta 3 and beta 4 integrins in the microvascular changes of psoriatic lesions.
大量证据表明，银屑病中观察到的微血管变化是血管增殖的结果。导致新血管形成的一系列事件中的关键步骤涉及内皮细胞与细胞外基质蛋白之间的相互作用，部分由整合素家族的黏附分子介导。已显示多种内皮整合素参与新血管形成，包括β1、β3和β4亚家族的成员。为了研究这些整合素在银屑病中的作用，从10例患有活动性、未经治疗的斑块状疾病的患者身上获取了皮损和非皮损皮肤标本。用针对α2、α5、α6、β1、αvβ3和β4整合素的单克隆抗体标记血管内皮。图像分析的使用允许对免疫过氧化物酶染色进行定量，并比较皮损和非皮损皮肤中的内皮标记。与非皮损皮肤相比，皮损中αvβ3整合素的内皮染色在浅表和深部血管中均显著增加。相比之下，与非皮损浅表真皮血管相比，皮损中内皮β4染色显著减少，α2、α5、α6和β1染色在两组之间无显著差异。这些结果证明了αvβ3和β4整合素在银屑病皮损微血管变化中的重要作用。
