Pérez-Cárceles M D, Osuna E, Vieira D N, Luna A
Department of Forensic Medicine, School of Medicine, University of Murcia, Spain.
Int J Legal Med. 1995;108(1):14-8. doi: 10.1007/BF01845610.
In some situations the postmortem diagnosis of myocardial infarction is made difficult by the brief course of the fatal episode or by interferences caused by autolysis. In such cases, biochemical indices may provide a useful adjunct to morphological studies. Myosin is the main component of the contractile apparatus of muscle cells, so its determination may well be useful to evaluate myocardial injury. The purpose of the present study was to establish the diagnostic efficacy of postmortem myosin heavy chain determinations using monoclonal antibodies and to compare this data with structural findings used to diagnose acute myocardial ischaemia. We studied 105 cadavers with a mean age of 61.63 +/- 2.21 years. Cases were allocated to 1 of 7 diagnostic groups depending on the probable intensity of myocardial damage and cause of death. The highest serum and pericardial fluid values of myosin heavy chains were seen in subjects who showed morphological evidence of myocardial ischaemia. Mean pericardial fluid/serum ratios differed significantly between subjects with and without observable signs of heart damage.
在某些情况下，致命事件病程短暂或自溶造成干扰，使得心肌梗死的尸检诊断变得困难。在此类病例中，生化指标可为形态学研究提供有用的辅助。肌球蛋白是肌肉细胞收缩装置的主要成分，因此其测定对于评估心肌损伤可能很有用。本研究的目的是确定使用单克隆抗体进行尸检肌球蛋白重链测定的诊断效能，并将此数据与用于诊断急性心肌缺血的结构发现进行比较。我们研究了105具尸体，平均年龄为61.63±2.21岁。根据心肌损伤的可能强度和死亡原因，将病例分为7个诊断组中的1组。在显示心肌缺血形态学证据的受试者中，肌球蛋白重链的血清和心包液值最高。有和没有明显心脏损伤迹象的受试者之间，心包液/血清平均比值存在显著差异。
