蜡样芽孢杆菌新溶血素基因的克隆与一级结构
Cloning and primary structure of a new hemolysin gene from Bacillus cereus.
作者信息
Baida G E, Kuzmin N P
机构信息
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
出版信息
Biochim Biophys Acta. 1995 Nov 7;1264(2):151-4. doi: 10.1016/0167-4781(95)00150-f.
A new hemolysin gene from Bacillus cereus VKM-B164 was cloned in Escherichia coli and sequenced. Deduced protein consists of 219 amino acids and has a molecular mass of 24.4 kDa. It has been concluded that the hemolysin, named 'hemolysin III', is distinct from the B. cereus hemolysins reported previously: cereolysin, sphingomyelinase, cereolysin AB, hemolysin II, and 'cereolysin-like' hemolysin (Honda, T., Shiba, A., Seo, S., Yamamoto, J., Matsuyama, J. and Miwatani, T. (1991) FEMS Microbiol. Lett. 79, 205-210).
从蜡样芽孢杆菌VKM - B164中分离出一个新的溶血素基因，并在大肠杆菌中进行克隆和测序。推导的蛋白质由219个氨基酸组成，分子量为24.4 kDa。已得出结论，这种名为“溶血素III”的溶血素与先前报道的蜡样芽孢杆菌溶血素不同：蜡样芽孢杆菌溶血素、鞘磷脂酶、蜡样芽孢杆菌溶血素AB、溶血素II和“类蜡样芽孢杆菌溶血素”（本田，T.，柴田，A.， 濑尾，S.，山本，J.，松山，J.和三渡谷，T.（1991年）FEMS微生物学快报79，205 - 210）。
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