Creyghton W M, de Waard-Siebinga I, Danen E H, Luyten G P, van Muijen G N, Jager M J
Department of Ophthalmology, University Hospital Leiden, The Netherlands.
Melanoma Res. 1995 Aug;5(4):235-42. doi: 10.1097/00008390-199508000-00005.
Adhesion molecules are likely to play a role in the process of tumour progression. We investigated the expression of integrins, ICAM-1, and CD44 and the influence of interferon-alpha (IFN-alpha), interferon-gamma (IFN-gamma), and tumour necrosis factor-alpha (TNF-alpha) on expression of these molecules on four uveal melanoma cell lines. The in vitro integrin expression was quite variable. The alpha V and beta 1 subunits were expressed on all cell lines, and none of the cell lines showed any alpha 3, beta 2, or beta 4 expression. Other integrin subunits showed a more variable pattern. ICAM-1 and CD44 were strongly expressed on all cell lines. IFN-alpha, IFN-gamma, and TNF-alpha upregulated alpha 1, alpha 2, and alpha 3 expression, and did not alter alpha 4, alpha 5, alpha 6, beta 2, alpha v beta 3, and beta 4 expression. The effects on alpha V and alpha V beta 5 were variable. ICAM-1 was upregulated by IFN-gamma and TNF-alpha, but not by IFN-alpha. Cytokine treatment hardly changed CD44 expression. In one case a comparison was made between expression on cultured cells and on tissue sections of the tumour of origin. Differences in expression were observed for the integrin subunits alpha 2, alpha 3, and alpha 5. This study shows that integrins and ICAM-1 expression on uveal melanoma cells in vitro are susceptible to cytokine treatment, but that the effects on integrin expression are cytokine and cell line dependent. Furthermore, some differences in integrin expression between cells in vivo and in vitro exist.
黏附分子可能在肿瘤进展过程中发挥作用。我们研究了整合素、细胞间黏附分子-1（ICAM-1）和CD44的表达，以及α干扰素（IFN-α）、γ干扰素（IFN-γ）和肿瘤坏死因子-α（TNF-α）对这四种葡萄膜黑色素瘤细胞系中这些分子表达的影响。体外整合素表达差异很大。αV和β1亚基在所有细胞系中均有表达，且没有细胞系显示出任何α3、β2或β4的表达。其他整合素亚基表现出更具变异性的模式。ICAM-1和CD44在所有细胞系中均强烈表达。IFN-α、IFN-γ和TNF-α上调了α1、α2和α3的表达，并且未改变α4、α5、α6、β2、αvβ3和β4的表达。对αV和αVβ5的影响各不相同。ICAM-1被IFN-γ和TNF-α上调，但未被IFN-α上调。细胞因子处理几乎未改变CD44的表达。在一个案例中，对培养细胞与原发肿瘤组织切片上的表达进行了比较。观察到整合素亚基α2、α3和α5在表达上存在差异。这项研究表明，体外葡萄膜黑色素瘤细胞上的整合素和ICAM-1表达易受细胞因子处理的影响，但对整合素表达的影响取决于细胞因子和细胞系。此外，体内和体外细胞之间在整合素表达上存在一些差异。
