Nakayama J, Guan X C, Nakashima M, Mashino T, Hori Y
Department of Dermatology, Kyushu University Faculty of Medicine, Fukuoka, Japan.
J Dermatol. 1997 Jun;24(6):351-60. doi: 10.1111/j.1346-8138.1997.tb02805.x.
The expression of intercellular adhesion molecule-1 (ICAM-1) in mouse B16 and human melanoma cell lines was investigated by indirect immunofluorescence using a FACScan analyzer. Mouse B16 melanoma cell lines (B16-F1 and F10) did not express ICAM-1 under ordinary culture conditions. Neither in vitro hyperthermia at 41 degrees C for 3 or 6 hr nor cytokines such as gamma interferon (IFN-gamma) or tumor necrosis factor alpha (TNF-alpha) induced ICAM-1 expression in B16 melanoma cell lines. A combination of IFN-gamma with TNF-alpha caused slight induction of ICAM-1 expression in the B16-F10 melanoma cell line. Hyperthermia at 41 degrees C for 3 hr in combination with the cytokines induced a slight expression of ICAM-1 in the B16-F1 melanoma cell line. Hyperthermia at 41 degrees C for 3 hr or 6 hr did not induce de novo ICAM-1 expression but hyperthermia at 43 degrees C for 6 hr caused rather suppression of the expression of ICAM-1 in the three human melanoma cell lines tested. In contrast, they showed a clear increase in the expression of ICAM-1 after treatment with either with IFN-gamma or TNF-alpha, and the expression was further augmented by a combination of the two cytokines. Treatment with cytokines in combination with hyperthermia at 41 degrees C or 43 degrees C for 3 hr did not augment the expression of ICAM-1 over that in cytokine-treated human melanoma cell lines, at normal temperatures. Thus, it is concluded that mouse B16-F1 and F10 melanoma cell lines are different from human melanoma cell lines in terms of induction of ICAM-1 expression by cytokines and/or hyperthermia.
采用FACScan分析仪通过间接免疫荧光法研究了细胞间黏附分子-1（ICAM-1）在小鼠B16和人黑色素瘤细胞系中的表达情况。小鼠B16黑色素瘤细胞系（B16-F1和F10）在普通培养条件下不表达ICAM-1。41℃体外热疗3小时或6小时，以及γ干扰素（IFN-γ）或肿瘤坏死因子α（TNF-α）等细胞因子均未诱导B16黑色素瘤细胞系表达ICAM-1。IFN-γ与TNF-α联合使用可使B16-F10黑色素瘤细胞系中ICAM-1表达略有增加。41℃热疗3小时联合细胞因子可使B16-F1黑色素瘤细胞系中ICAM-1略有表达。41℃热疗3小时或6小时未诱导ICAM-1从头表达，但43℃热疗6小时导致所检测的三种人黑色素瘤细胞系中ICAM-1表达受到抑制。相反，用IFN-γ或TNF-α处理后，它们的ICAM-1表达明显增加，两种细胞因子联合使用可进一步增强其表达。细胞因子与41℃或43℃热疗3小时联合处理并未使ICAM-1表达高于常温下细胞因子处理的人黑色素瘤细胞系。因此，得出结论，小鼠B16-F1和F10黑色素瘤细胞系在细胞因子和/或热疗诱导ICAM-1表达方面与人黑色素瘤细胞系不同。
