Klavins J V, Wessely Z, Pickett P
Ann Clin Lab Sci. 1978 Jan-Feb;8(1):74-8.
To obtain as much information as possible from light-microscopy about changes in the glomerular basement-membrane, a thin section microtome attachment is necessary in order to produce 1 to 3 micron sections. The most informative staining method of paraffin embedded tissue is the Muller-Mowry-Masson trichrome (M-M-M) combination stain. The deposits, e.g., in systemic lupus erythematosus (SLE), stain red, the basement membrane light orange and the endothelial cell cytoplasm light pink. With this stain, as with the Muller-Mowry stain, normal glomeruli show an uninterrupted line of blue acid mucopolysaccharides along the basement membrane, where it is joined by foot processes of the epithelial cells. In contrast, in glomeruli from patients with SLE, focal loss of acid mucopolysaccharides is noted.
为了从光学显微镜中获取尽可能多的关于肾小球基底膜变化的信息，需要一个薄切片切片机附件来制作1至3微米厚的切片。石蜡包埋组织最具信息量的染色方法是穆勒-莫里-马森三色（M-M-M）联合染色。例如，在系统性红斑狼疮（SLE）中，沉积物染成红色，基底膜呈浅橙色，内皮细胞胞质呈浅粉色。用这种染色方法，与穆勒-莫里染色一样，正常肾小球在基底膜处显示一条不间断的蓝色酸性粘多糖线，此处有上皮细胞的足突与之相连。相比之下，在SLE患者的肾小球中，可观察到酸性粘多糖的局灶性缺失。
