针对重组伤寒沙门氏菌36千道尔顿孔蛋白的沙门氏菌特异性单克隆抗体。
Salmonella-specific monoclonal antibodies against recombinant Salmonella typhi 36-kilodalton porin.
作者信息
Kissel V, Gonzalez C, Astudillo M, Godard A, Wachman B, Cabello F C
机构信息
Department of Microbiology and Immunology, New York Medical College, Valhalla 10595, USA.
出版信息
Clin Diagn Lab Immunol. 1994 Mar;1(2):250-2. doi: 10.1128/cdli.1.2.250-252.1994.
Mouse monoclonal antibodies were raised against recombinant Salmonella typhi 36-kDa porin monomer. Specificities of 16 monoclonal antibodies were analyzed as reactivity patterns in dot immunobinding and Western blot (immunoblot) assays using isolated outer membrane proteins of gram-negative bacteria and cloned purified S. typhi porin monomers and trimers. Four monoclonal antibodies were specific for Salmonella spp.
制备了针对重组伤寒沙门氏菌36-kDa孔蛋白单体的小鼠单克隆抗体。使用革兰氏阴性菌的分离外膜蛋白以及克隆纯化的伤寒沙门氏菌孔蛋白单体和三聚体，通过斑点免疫结合和蛋白质印迹（免疫印迹）分析了16种单克隆抗体的特异性，作为反应模式。四种单克隆抗体对沙门氏菌属具有特异性。
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