Johnstone J J, Bitensky L, Cashman B, Chayen J
Ann Rheum Dis. 1978 Dec;37(6):552-6. doi: 10.1136/ard.37.6.552.
To examine the reported beneficial effect of zinc in rheumatoid arthritis, rheumatoid synovial tissue has been maintained in vitro in non-proliferative culture with or without zinc sulphate in the culture medium. Alkaline phosphatase activity was measured by microdensitometry of the cytochemical reaction in cryostat sections; the activity in blood vessels was measured separately from that in the supporting tissue below the synovial surface. Zinc enhanced this activity optimally at concentrations of between 10(-5) and 10(-4) mol/l.
为研究锌在类风湿性关节炎中所报道的有益作用，类风湿性滑膜组织在体外非增殖性培养条件下，于培养基中添加或不添加硫酸锌进行培养。通过对低温恒温器切片中细胞化学反应的显微密度测定法来测量碱性磷酸酶活性；血管中的活性与滑膜表面下方支持组织中的活性分开测量。锌在浓度为10⁻⁵至10⁻⁴摩尔/升之间时能最佳地增强这种活性。
