al-Sabti K
Jozef Stefan Institute, Ljubljana, Slovenia.
Cytobios. 1995;82(330):181-7.
The use of two cytochalasin-blocked methods with erythrocyte and hepatic cells was developed to identify spontaneous and/or induced triploid fish by observing the presence of trinucleated cells. Spontaneous triploids were found in two specimens out of fifteen commercial rainbow trout from a hatchery at 2 years of age. Some rare spontaneous triploid (3n) trinucleated cells were also found in the other thirteen diploid (2n) trout specimens under investigation. The use of this novel technique in a study of the triploidy in vitro, allows evaluation of the polyploid specimens under field and laboratory experimental conditions. The potential contribution of binucleated hepatic and erythrocyte techniques in fish to the assessment of polyploidy is discussed.
开发了两种使用细胞松弛素阻断的方法，分别作用于红细胞和肝细胞，通过观察三核细胞的存在来鉴定自发和/或诱导的三倍体鱼类。在一个孵化场的15条2岁商业虹鳟鱼样本中，有2条发现了自发三倍体。在另外13条正在研究的二倍体（2n）鳟鱼样本中也发现了一些罕见的自发三倍体（3n）三核细胞。在一项关于三倍体体外研究中使用这种新技术，可以在野外和实验室实验条件下评估多倍体样本。讨论了双核肝细胞和红细胞技术在鱼类多倍体评估中的潜在作用。
