Lukomska B, Olszewski W L
Arch Immunol Ther Exp (Warsz). 1978;26(1-6):423-8.
Preservation of liver for transplantation into allogeneic recipient affects the integrity and function of Kupffer cells. Rapid method for obtaining pure macrophages by dispersing the liver with collagenase and pronase would open possibility for different investigations. The experiments concerning morphology and phagocytic function were performed on Kupffer cells after 21 hours in vitro culture in Eagle medium with 20% inactivated calf serum. Studies on endocytosis using human red cells and colloidal carbon indicated that Kupffer cells revealed high phagocytic activity after culture.
用于移植到异体受体的肝脏保存会影响库普弗细胞的完整性和功能。通过用胶原酶和链霉蛋白酶分散肝脏来获得纯巨噬细胞的快速方法将为不同的研究开辟可能性。在用含20%灭活小牛血清的伊格尔培养基进行体外培养21小时后，对库普弗细胞进行了有关形态学和吞噬功能的实验。使用人红细胞和胶体碳进行的内吞作用研究表明，培养后的库普弗细胞显示出高吞噬活性。
