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[人库普弗细胞的分离与培养]

[Isolation and culture of human Kupffer cells].

作者信息

Kirn A, Steffan A M, Bingen A, Cinqualbre J, Gendrault J L

出版信息

C R Seances Acad Sci D. 1980 Sep 15;291(2):249-51.

PMID:6775836
Abstract

The dissociation of human adult liver parenchyma by collagenase followed by cell separation with centrifugal elutriation allows the Kupffer cells to be isolated. These cells, which may be maintained in culture, retain their main morphological and functional features.

摘要

用胶原酶解离成人肝脏实质,然后通过离心淘洗进行细胞分离,可分离出库普弗细胞。这些细胞可在培养中维持,保留其主要的形态和功能特征。

相似文献

1
[Isolation and culture of human Kupffer cells].[人库普弗细胞的分离与培养]
C R Seances Acad Sci D. 1980 Sep 15;291(2):249-51.
2
[Rat Kupffer cells cultures and their applications to experimental hepatology (author's transl)].[大鼠库普弗细胞培养及其在实验性肝脏病学中的应用(作者译)]
Nouv Presse Med. 1980;9(31):2137-40.
3
Isolation and identification of rat Kupffer cells.大鼠库普弗细胞的分离与鉴定。
Arch Immunol Ther Exp (Warsz). 1978;26(1-6):423-8.
4
Ultrastructure of human Kupffer cells maintained in culture.
J Submicrosc Cytol Pathol. 1995 Apr;27(2):161-70.
5
Morphological characterization of Kupffer and endothelial cells of rat liver isolated by counterflow elutriation.通过逆流淘析法分离的大鼠肝脏库普弗细胞和内皮细胞的形态学特征
Gastroenterology. 1978 Jul;75(1):80-7.
6
Surface features and ultrastructure of isolated Kupffer cells as seen by scanning and transmission electron microscopy.通过扫描电子显微镜和透射电子显微镜观察分离的库普弗细胞的表面特征和超微结构。
Blood Cells. 1978;4(1-2):301-18.
7
[Improving the ways to isolate, cultivate and identify rat Kupffer cells].[改进大鼠库普弗细胞的分离、培养及鉴定方法]
Zhonghua Gan Zang Bing Za Zhi. 2006 Jul;14(7):532-5.
8
Evidence of macrophage receptors capable of direct recognition of xenogeneic epitopes without opsonization.巨噬细胞受体能够在无调理作用的情况下直接识别异种抗原表位的证据。
Xenotransplantation. 2005 Jan;12(1):13-9. doi: 10.1111/j.1399-3089.2004.00195.x.
9
[Mixed enzyme applied to develop the method on BAlb/c mouse Kupffer cell isolated and cultured in vitro].[混合酶应用于体外分离培养BAlb/c小鼠库普弗细胞方法的建立]
Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Mar;39(2):298-301.
10
Isolated hepatic lipocytes and Kupffer cells from normal human liver: morphological and functional characteristics in primary culture.来自正常人类肝脏的分离肝星状细胞和库普弗细胞:原代培养中的形态学和功能特征
Hepatology. 1992 Feb;15(2):234-43. doi: 10.1002/hep.1840150211.

引用本文的文献

1
Infection of primary cultures of human Kupffer cells by Dengue virus: no viral progeny synthesis, but cytokine production is evident.登革病毒对人库普弗细胞原代培养物的感染:无病毒子代合成,但细胞因子产生明显。
J Virol. 1999 Jun;73(6):5201-6. doi: 10.1128/JVI.73.6.5201-5206.1999.
2
Intrasplenic immunization with infected hepatocytes: a mouse model for studying protective immunity against malaria pre-erythrocytic stage.用感染的肝细胞进行脾内免疫:一种研究针对疟疾红细胞前期保护性免疫的小鼠模型。
Immunology. 1994 May;82(1):164-8.