Kirn A, Steffan A M, Bingen A, Cinqualbre J, Gendrault J L
C R Seances Acad Sci D. 1980 Sep 15;291(2):249-51.
The dissociation of human adult liver parenchyma by collagenase followed by cell separation with centrifugal elutriation allows the Kupffer cells to be isolated. These cells, which may be maintained in culture, retain their main morphological and functional features.
用胶原酶解离成人肝脏实质,然后通过离心淘洗进行细胞分离,可分离出库普弗细胞。这些细胞可在培养中维持,保留其主要的形态和功能特征。