甲型流感病毒的纯化及通过聚丙烯酰胺凝胶电泳（PAGE）对病毒蛋白的初步表征。
Purification of influenza A virus and preliminary characterization of virus proteins by polyacrylamide gel electrophoresis (PAGE).
作者信息
Chomik M
出版信息
Arch Immunol Ther Exp (Warsz). 1978;26(1-6):537-40.
PMID:749805
Abstract
Influenza A/ZSSR/053/74 (H3N2) from egg ellantoic fluid was partially purified by absorption and elution on chick erythrocytes and chromatography on Sepharose 2B. Concentrated and partially purified preparation of virus containing 5000 HAV and 80 micrograms of protein per ml was subjected to PAGE in 0.1% SDS. Eleven protein bands were selected of which three were identified as NA, NP and HA.
摘要
从鸡蛋尿囊液中提取的甲型流感病毒/苏联/053/74（H3N2）通过在鸡红细胞上的吸附与洗脱以及在琼脂糖2B上的层析进行部分纯化。对每毫升含有5000个血凝单位（HAU）和80微克蛋白质的浓缩且部分纯化的病毒制剂进行0.1%十二烷基硫酸钠（SDS）聚丙烯酰胺凝胶电泳（PAGE）。选取了11条蛋白带，其中3条被鉴定为神经氨酸酶（NA）、核蛋白（NP）和血凝素（HA）。
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