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一维采用固定化pH梯度的二维聚丙烯酰胺凝胶电泳（IPG-Dalt）：技术现状以及垂直系统与水平系统的争议

Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt): the state of the art and the controversy of vertical versus horizontal systems.

作者信息

Görg A, Boguth G, Obermaier C, Posch A, Weiss W

机构信息

Technical University of Munich, Institute of Food Technology, Freising-Weihenstephan.

出版信息

Electrophoresis. 1995 Jul;16(7):1079-86. doi: 10.1002/elps.11501601183.

DOI:10.1002/elps.11501601183
PMID:7498150
Abstract

After having established the basic protocol of two-dimensional electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt) in 1988 (A. Görg et al., Electrophoresis 1988, 9, 531-546), some critical parameters of the actual IPG-Dalt protocols as well as the results obtained with horizontal and vertical second-dimensional sodium dodecyl sulfate-electrophoresis are demonstrated and discussed.

摘要

1988年建立了在第一维使用固定化pH梯度的二维电泳基本方案（IPG-Dalt）（A. Görg等人，《电泳》，1988年，第9卷，531 - 546页）之后，本文展示并讨论了实际IPG-Dalt方案的一些关键参数，以及水平和垂直二维十二烷基硫酸钠电泳所获得的结果。

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Two-dimensional polyacrylamide gel electrophoresis, with immobilized pH gradients in the first dimension, of barley seed proteins: discrimination of cultivars with different malting grades.采用第一向为固定化pH梯度的二维聚丙烯酰胺凝胶电泳分析大麦种子蛋白：区分不同制麦等级的品种。
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Recent developments in two-dimensional gel electrophoresis with immobilized pH gradients: wide pH gradients up to pH 12, longer separation distances and simplified procedures.固定化pH梯度二维凝胶电泳的最新进展：高达pH 12的宽pH梯度、更长的分离距离和简化的操作程序。
Electrophoresis. 1999 Apr-May;20(4-5):712-7. doi: 10.1002/(SICI)1522-2683(19990101)20:4/5<712::AID-ELPS712>3.0.CO;2-Y.
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Positional reproducibility of protein spots in two-dimensional polyacrylamide gel electrophoresis using immobilised pH gradient isoelectric focusing in the first dimension: an interlaboratory comparison.在二维聚丙烯酰胺凝胶电泳中使用固定化pH梯度等聚焦法进行第一维分离时蛋白质斑点的位置重现性：一项实验室间比较
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Horizontal SDS-PAGE for IPG-Dalt.用于IPG-Dalt的水平SDS-聚丙烯酰胺凝胶电泳
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Two-dimensional gel electrophoretic analysis with immobilized pH gradients of Dactylis glomerata pollen allergens.利用固定化pH梯度对鸭茅花粉过敏原进行二维凝胶电泳分析。
Int Arch Allergy Immunol. 1993;102(1):72-80. doi: 10.1159/000236553.

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