多种小肠结肠炎耶尔森菌抗原用于被动血凝试验的效用。

The usefulness of various Yersinia enterocolitica antigens for passive hemagglutination test.

作者信息

Zaremba M

出版信息

Arch Immunol Ther Exp (Warsz). 1978;26(1-6):625-30.

Abstract

Using various antigen preparations of seven Yersinia enterocolitica serotypes (according to Knapp and Thal) and homologous rabbit immune sera the author succeeded in standardization of passive hemagglutination test. The usefulness of preparations termed "supernatant" derived from heated cells at 100 degrees C and lyophilized "crude" preparations of all serotypes were assessed using blood donors' sera and sera of subjects suspected of infection with Y. enterocolitica. It has been shown that though the diagnostic value of supernatant is higher than that of crude fraction, with the former one it is not possible to differentiate hemagglutinins against particular serotypes. More detailed assessment has been limited to serotype IA, only. An equal usefulness for passive hemagglutination of preparations termed "CA free crude" and "ethanol insoluble fraction" derived from TCA extracted cell sediment has been found.

摘要

作者使用七种小肠结肠炎耶尔森菌血清型（根据克纳普和塔尔的分类）的各种抗原制剂以及同源兔免疫血清，成功实现了被动血凝试验的标准化。使用献血者血清和疑似感染小肠结肠炎耶尔森菌患者的血清，对在100℃加热细胞得到的称为“上清液”的制剂以及所有血清型的冻干“粗制”制剂的效用进行了评估。结果表明，尽管上清液的诊断价值高于粗制部分，但使用前者无法区分针对特定血清型的血凝素。更详细的评估仅局限于IA血清型。已发现从三氯乙酸提取的细胞沉淀物中得到的称为“无CA粗制品”和“乙醇不溶部分”的制剂在被动血凝试验中的效用相同。

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引用本文的文献

1

[Determination of O-agglutinins against Yersinia enterocolitica by the indirect hemagglutination test (author's transl)].用间接血凝试验测定抗小肠结肠炎耶尔森菌的O凝集素（作者译）

Klin Wochenschr. 1981 May 15;59(10):517-9. doi: 10.1007/BF01696214.

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