Kiyosawa I, Aoki M, Imamura T, Nagasawa T, Ito T, Sibata N, Naito J, Saito T R, Takahashi K W
Kissei Pharmaceutical Co., Ltd., Nagano, Japan.
Exp Anim. 1995 Oct;43(5):651-61. doi: 10.1538/expanim1978.43.5_651.
Male rats of the WBN/Kob strain, which are known to spontaneously develop diabetes with aging, were examined for histopathological changes in the retina. Five rats (10 eyes) each of WBN/Kob and Wistar/ST as a control were used, and the thickness of the retinal layers, both the central region and the peripheral region of the retina, were measured on weeks 1, 2, 3, 5, 9, 13, 17, 23, 27, 36, 45, 54, 67 and 80 after birth. The rod and cone cell layer in WBN/Kob rats was under-grown, and its thickness decreased 71.7% in the central zone and 59.3% in the peripheral zone of the retina compared with that of the control. In the central and peripheral retina, the rod and cone cell layer, outer nuclear layer and outer plexiform layer, as well as the inner plexiform layer in the central retina, gradually decreased in thickness from 5-45 weeks of age. In the central and peripheral parts of the retina, the number of nuclei decreased in accordance with the thinning of the nuclear layer. The thinned layers showed only cell loss. The rod and cone cell layer of the peripheral retina was thinner than that of the central retina. We obtained the following findings in the retina of male WBN/Kob rats. First, the rod and cone cell layer is undergrown compared with that of the control. Second, the first change occurred in the rod and cone cell layer. Third, the thinning of the rod and cone cell layer appeared at 5 weeks of age, and thinning with aging was slow. And finally, the thinning of the peripheral retina was more severe than that of the central retina. From the above findings, it seems that retinal changes in WBN/Kob rats are similar to the retinal degeneration of rds mice (retinal degeneration slow mice) and that WBN/Kob rats provide a useful animal model for human retinopathy.
已知WBN/Kob品系的雄性大鼠会随着年龄增长自发患上糖尿病，对其视网膜进行了组织病理学变化检查。使用5只WBN/Kob大鼠（10只眼睛）和作为对照的5只Wistar/ST大鼠，在出生后的第1、2、3、5、9、13、17、23、27、36、45、54、67和80周测量视网膜中央区域和周边区域各层的厚度。WBN/Kob大鼠的视杆和视锥细胞层发育不良，与对照组相比，其厚度在视网膜中央区减少了71.7%，在周边区减少了59.3%。在视网膜中央和周边区域，视杆和视锥细胞层、外核层和外网状层，以及中央视网膜的内网状层，在5至45周龄时厚度逐渐减小。在视网膜中央和周边部分，细胞核数量随着核层变薄而减少。变薄的层仅显示细胞丢失。周边视网膜的视杆和视锥细胞层比中央视网膜的薄。我们在雄性WBN/Kob大鼠的视网膜中获得了以下发现。第一，视杆和视锥细胞层与对照组相比发育不良。第二，最早的变化发生在视杆和视锥细胞层。第三，视杆和视锥细胞层在5周龄时开始变薄，并且随着年龄增长变薄速度缓慢。最后，周边视网膜的变薄比中央视网膜更严重。从上述发现来看，WBN/Kob大鼠的视网膜变化似乎与rds小鼠（视网膜变性缓慢小鼠）的视网膜变性相似，并且WBN/Kob大鼠为人类视网膜病变提供了一个有用的动物模型。
