Kiyosawa I, Aoki M, Imamura T, Shibata N, Naito J, Saito T R, Takahashi K W
Kissei Pharmaceutical Co., Ltd., Nagano, Japan.
Jikken Dobutsu. 1994 Jul;43(3):357-67. doi: 10.1538/expanim1978.43.3_357.
Changes in the visual function of the male WBN/Kob rat known as diabetic strain with increasing age were studied electrophysiologically. The measurements of electroretinogram (ERG) and visual evoked potential (VEP) in male WBN/Kob rats were performed at 5, 9, 13, 17, 23, 27, 36, 45, 54, 67 and 80 weeks of age, and compared with those in male Wistar/ST rats used as control. The prolonged latencies and decreased amplitudes of a- and b- waves and oscillatory potentials of ERG in WBN/Kob rats aged over 17 or 27 weeks were recorded under the light or after 20 min dark adaptation. The visual abnormality as indicated by non-recordable ERG was obvious in WBN/Kob rats aged 67 and 80 weeks. No changes of N1, P and N2 peak latencies of VEP were recorded in WBN/Kob rats with increasing age. The amplitudes between N1 peaks and P peaks decreased remarkably in WBN/Kob rats aged 54 weeks. The amplitudes of VEP in 67 or 80 weeks old rats in which ERG disappeared showed 26 or 28% of those in 5 weeks old rats. From these results, recording of ERG and VEP suggested that the visual abnormality occurred in male WBN/Kob rats aged 67 or 80 weeks and the process of visual abnormality with increasing age was clarified.
对被称为糖尿病品系的雄性WBN/Kob大鼠随着年龄增长视觉功能的变化进行了电生理学研究。在5、9、13、17、23、27、36、45、54、67和80周龄时对雄性WBN/Kob大鼠进行视网膜电图（ERG）和视觉诱发电位（VEP）测量，并与作为对照的雄性Wistar/ST大鼠进行比较。在光照下或暗适应20分钟后，记录了17周或27周龄以上WBN/Kob大鼠ERG的a波和b波以及振荡电位的潜伏期延长和振幅降低情况。在67周和80周龄的WBN/Kob大鼠中，ERG不可记录所表明的视觉异常很明显。随着年龄增长，WBN/Kob大鼠的VEP的N1、P和N2峰潜伏期没有变化。在54周龄的WBN/Kob大鼠中，N1峰和P峰之间的振幅显著降低。在ERG消失的67周或80周龄大鼠中，VEP的振幅分别为5周龄大鼠的26%或28%。从这些结果来看，ERG和VEP的记录表明67周或80周龄的雄性WBN/Kob大鼠出现了视觉异常，并且随着年龄增长视觉异常的过程得以阐明。
