Ueda O, Kamada N, Suzuki H
CSK Research Park, Inc., Shizuoka, Japan.
Exp Anim. 1995 Oct;43(5):679-85. doi: 10.1538/expanim1978.43.5_679.
To define the developmental fate of two different stages of embryos existing together in the mouse reproductive tract, different stages of fresh and frozen-thawed embryos were transferred separately into the oviducts of identical recipients. ICR and FvB embryos were flushed from the oviducts and/or uterus of superovulated females on Day 1 (2-4-cell stage) and Day 2 (8-cell-morula stage) of pregnancy. Day 1 embryos were transferred separately to the right or left oviduct of recipients, while the other oviduct received Day 2 embryos. There were no significant differences between Day 1 and Day 2 embryos with respect to the number of implantation sites and live fetuses in either the fresh or frozen-thawed embryos. These results concerning to the embryonic stage were similar to those of control experiments, in which Day 1 or Day 2 embryos were transferred to both right and left oviducts of recipients. Furthermore, no strain differences were observed in this study. No developmental retardation or anomalies were observed in fetuses derived from either Day 1 or Day 2 embryos. The embryo transfer in this study revealed that differences in developmental stage at preimplantation were synchronized by the maternal uterine environment.
为了确定共同存在于小鼠生殖道中的两个不同发育阶段胚胎的发育命运，将新鲜和冻融胚胎的不同阶段分别移植到相同受体的输卵管中。在妊娠第1天（2-4细胞期）和第2天（8细胞-桑椹胚期），从超排卵雌性小鼠的输卵管和/或子宫中冲洗出ICR和FvB胚胎。第1天的胚胎分别移植到受体的右侧或左侧输卵管，而另一侧输卵管接受第2天的胚胎。在新鲜或冻融胚胎中，第1天和第2天的胚胎在着床部位数量和活胎数量方面没有显著差异。这些关于胚胎阶段的结果与对照实验相似，在对照实验中，第1天或第2天的胚胎被移植到受体的左右两侧输卵管。此外，本研究未观察到品系差异。在源自第1天或第2天胚胎的胎儿中未观察到发育迟缓或异常。本研究中的胚胎移植表明，着床前发育阶段的差异通过母体子宫环境得以同步。
