神经节苷脂GM1的一种光反应性衍生物特异性地在细胞表面交联VIP21-小窝蛋白。

A photo-reactive derivative of ganglioside GM1 specifically cross-links VIP21-caveolin on the cell surface.

作者信息

Fra A M, Masserini M, Palestini P, Sonnino S, Simons K

机构信息

European Molecular Biology Laboratory, Heidelberg, Germany.

出版信息

FEBS Lett. 1995 Nov 13;375(1-2):11-4. doi: 10.1016/0014-5793(95)95228-o.

DOI:10.1016/0014-5793(95)95228-o

PMID:7498456

Abstract

Previous studies have shown that sphingolipids may be enriched in caveolae, plasmalemmal invaginations implicated in endocytosis and signal transduction. We synthesised a radiolabeled derivative of ganglioside GM1 bearing a photo-reactive cross-linker at the end of its acyl chain. After insertion in the plasma membrane of cultured A431 or MDCK cells and photoactivation, the main protein cross-linked by the GM1 derivative was VIP21-caveolin, an essential structural component of caveolae. This result shows close proximity between GM1 molecules and VIP21-caveolin in the caveolar membrane and strongly implicates sphingolipid segregation in the biogenesis of caveolae.

摘要

先前的研究表明，鞘脂类物质可能在小窝中富集，小窝是质膜内陷结构，与内吞作用和信号转导有关。我们合成了一种神经节苷脂GM1的放射性标记衍生物，其酰基链末端带有光反应性交联剂。将其插入培养的A431或MDCK细胞的质膜并进行光活化后，与GM1衍生物交联的主要蛋白质是VIP21-小窝蛋白，它是小窝的一种重要结构成分。这一结果表明，在小窝膜中GM1分子与VIP21-小窝蛋白靠得很近，有力地证明了鞘脂类物质在小窝生物发生过程中的分离作用。