Spyr C A, Käsermann F, Kempf C
Institute of Biochemistry, University of Bern, Switzerland.
FEBS Lett. 1995 Nov 13;375(1-2):134-6. doi: 10.1016/0014-5793(95)01197-m.
Pore formation at mildly acidic pH by SFV spike proteins was investigated using isolated and modified virions. Modification of the virions was performed by limited proteolysis in presence of octylglucoside and resulted in the formation of E1 particles and spikeless particles, respectively. Pore formation was detected by measuring the influx of propidium iodide into the viral particles. The results obtained clearly showed that the presence of E1 alone is sufficient to promote pore formation at mildly acidic pH. Thus E1 represents the pore forming element of the viral spike proteins.
利用分离和修饰的病毒粒子研究了辛德毕斯病毒（SFV）刺突蛋白在轻度酸性pH值下形成孔道的情况。在辛基葡糖苷存在下通过有限蛋白酶解对病毒粒子进行修饰，分别导致形成E1粒子和无刺突粒子。通过测量碘化丙啶流入病毒粒子来检测孔道形成。所获得的结果清楚地表明，仅E1的存在就足以促进在轻度酸性pH值下形成孔道。因此，E1代表病毒刺突蛋白的孔道形成元件。
