Kempf C, Michel M R, Omar A, Jentsch P, Morell A
Central Laboratory, Blood Transfusion Service, Swiss Red Cross.
Biosci Rep. 1990 Aug;10(4):363-74. doi: 10.1007/BF01117236.
Semliki Forest virus-induced cell-cell fusion from within was considered to exclusively occur at mildly acidic pH (less than 6.2). Data of this study show that such cell fusion can also be triggered by transient acidification of the cytoplasm of infected cells at an extracellular, neutral pH. Results were obtained by utilizing NH4Cl pulses combined with covalent modification of cell surface proteins. The observation implies a revision of the current consensus regarding the mechanism of Semliki Forest virus induced cell-cell fusion. We propose a model in which at least two peptide segments of the viral spike protein E1 may be involved in triggering the fusion event.
塞姆利基森林病毒诱导的细胞内细胞融合曾被认为仅在轻度酸性pH值（低于6.2）时发生。本研究数据表明，在细胞外中性pH值条件下，感染细胞细胞质的短暂酸化也可触发这种细胞融合。通过利用氯化铵脉冲结合细胞表面蛋白的共价修饰获得了相关结果。这一观察结果意味着对目前关于塞姆利基森林病毒诱导细胞融合机制的共识需要进行修正。我们提出了一个模型，其中病毒刺突蛋白E1的至少两个肽段可能参与触发融合事件。
