Dwyer T M, Froehner S C
Department of Physiology, University of North Carolina, Chapel Hill 27599-7545, USA.
FEBS Lett. 1995 Nov 13;375(1-2):91-4. doi: 10.1016/0014-5793(95)01176-f.
Syntrophin, a 58-kDa membrane-associated protein, is one component of a protein complex associated with dystrophin and other members of the dystrophin family, including the 87-kDa homologue (87K protein). To characterize interactions between synthrophin and 87K protein, we used an in vitro overlay binding assay. We demonstrate that purified Torpedo syntrophin binds directly to dystrophin and 87K. By expressing overlapping regions of the 87K protein as bacterial fusion proteins for binding targets, we show that a 52-amino acid region of 87K (residues 375-426) is sufficient for binding syntrophin.
肌养蛋白是一种58千道尔顿的膜相关蛋白，是与肌营养不良蛋白及肌营养不良蛋白家族其他成员（包括87千道尔顿同源物，即87K蛋白）相关的蛋白复合物的一个组成部分。为了表征肌养蛋白与87K蛋白之间的相互作用，我们采用了体外覆盖结合试验。我们证明，纯化的电鳐肌养蛋白可直接与肌营养不良蛋白及87K蛋白结合。通过将87K蛋白的重叠区域表达为用于结合靶点的细菌融合蛋白，我们发现87K蛋白的一个52个氨基酸的区域（第375 - 426位氨基酸残基）足以与肌养蛋白结合。
