Brasil M R, Collares E F, Soares F C
Arq Gastroenterol. 1978 Jul-Sep;15(3):141-5.
It was taken 32 male Wistar rats, weighting between 130 g and 150 g, free feeding, to study the total and specific activities of lactase, invertase and maltase of small intestine of rats. The animals were divided by chance in 3 experimental and 1 control group. 1. group--Aloxanic diabetes rats: treated with 1 unit of NPH insulin every day: after the 4th day of aloxane administration, all rats were killed. 2. group--Aloxanic diabetes rats--treated for 5 days with 1 unit of NPH insulin every day; after the 5th day until the 7th they were treated with 4 units of NPH insulin and were also killed. 3. group--Hyperinsulinism rats--Normal rats were treated for 4 days with 4 units of NPH insulin every day. After the 5th day they were killed. 4. group--Control group--Normal rats, free feeding. They were observed during 4 days and were also killed. The results showed that none difference was observed in the 4 groups of rats about the total and specific activities of lactase, invertase and maltase of the small intestine. In this study, all the animals with aloxanic diabetes were treated with insulin. Then, it is possible that the insulin inhibited the stimulator effect of the diabetes upon the dissacaridases of the small intestine of the rats.
选取32只体重在130克至150克之间、自由进食的雄性Wistar大鼠，用于研究大鼠小肠乳糖酶、蔗糖酶和麦芽糖酶的总活性和比活性。将动物随机分为3个实验组和1个对照组。1组——四氧嘧啶糖尿病大鼠：每天用1单位中效胰岛素治疗；在给予四氧嘧啶4天后，处死所有大鼠。2组——四氧嘧啶糖尿病大鼠——每天用1单位中效胰岛素治疗5天；在第5天至第7天，用4单位中效胰岛素治疗，然后也处死。3组——高胰岛素血症大鼠——正常大鼠每天用4单位中效胰岛素治疗4天。在第5天后处死。4组——对照组——正常大鼠，自由进食。观察4天后处死。结果表明，4组大鼠小肠乳糖酶、蔗糖酶和麦芽糖酶的总活性和比活性未观察到差异。在本研究中，所有四氧嘧啶糖尿病动物均用胰岛素治疗。那么，胰岛素有可能抑制了糖尿病对大鼠小肠双糖酶的刺激作用。
