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通过试管凝集试验和血凝抑制试验对火鸡支原体抗体进行的比较。

Comparison of Mycoplasma meleagridis antibodies demonstrated by tube agglutination and hemagglutination-inhibition tests.

作者信息

Rhoades K R

出版信息

Avian Dis. 1978 Oct-Dec;22(4):633-8.

PMID:749888
Abstract

Groups of turkeys inoculated intravenously with hemagglutinating (HA) and nonhemagglutinating (NHA) strains of Mycoplasma meleagridis developed similar antibody titers as demonstrated by the tube agglutination (TA) test, but use of the hemagglutination-inhibition (HI) test indicated markedly higher titers for turkeys inoculated with the HA strain than for those inoculated with the NHA strain. Adsorption of serum antibody with NHA strain antigen removed most agglutination activity but had little effect on HI activity. These results indicate that the HI and TA tests demonstrated antibody against different antigens, with the HI test detecting antibody directed against the hemagglutinin and the TA test detecting antibody directed against membrane antigens common to both strains.

摘要

用血凝性(HA)和非血凝性(NHA)火鸡支原体菌株静脉接种的火鸡群体,通过试管凝集(TA)试验显示出相似的抗体滴度,但血凝抑制(HI)试验表明,接种HA菌株的火鸡的滴度明显高于接种NHA菌株的火鸡。用NHA菌株抗原吸附血清抗体可去除大部分凝集活性,但对HI活性影响很小。这些结果表明,HI和TA试验检测的是针对不同抗原的抗体,HI试验检测针对血凝素的抗体,而TA试验检测针对两种菌株共有的膜抗原的抗体。

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