Kaszanyitzky E, Czifra G, Stipkovits L
Veterinary Institute of Debrecen, Hungary.
Acta Vet Hung. 1994;42(1):69-78.
Comparative examination of a total of 1,030 blood samples from six turkey flocks of three Eastern Hungarian turkey farms was performed by the conventional haemagglutination inhibition (HI) and slide agglutination (SA) tests and by a competitive ELISA visualizing the inhibition by a positive test serum of the reaction between a monoclonal antibody and the specific epitope of Mycoplasma gallisepticum recognized by it. All the three tests detected the flocks which were certainly infected. The highest rate of positivity (93% of the samples tested) was revealed by the ELISA. By SA and HI the positivity rate was 56% and 55%, respectively. Thirty-five per cent of the positive blood samples reacted in all three tests, 17% of them only by ELISA and HI, another 17% only by ELISA and SA, while 3% only by SA and HI. In the case of positive flocks first the SA test and ELISA, then the HI test and ELISA give parallel results.
对匈牙利东部三个火鸡养殖场的六个火鸡群共1030份血样进行了比较检测，采用传统的血凝抑制（HI）试验、玻片凝集（SA）试验以及一种竞争性酶联免疫吸附测定（ELISA），该ELISA可显示阳性试验血清对单克隆抗体与其所识别的鸡毒支原体特异性表位之间反应的抑制作用。所有这三种检测方法均能检测出确实感染的鸡群。ELISA检测出的阳性率最高（所检测样本的93%）。SA试验和HI试验的阳性率分别为56%和55%。35%的阳性血样在所有三种检测中均呈阳性反应，其中17%仅在ELISA和HI试验中呈阳性，另有17%仅在ELISA和SA试验中呈阳性，而3%仅在SA和HI试验中呈阳性。对于阳性鸡群，首先SA试验和ELISA的结果平行，然后HI试验和ELISA的结果平行。
