Chen Q, Olashaw N, Wu J
Molecular Oncology Program, H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida 33612, USA.
J Biol Chem. 1995 Dec 1;270(48):28499-502. doi: 10.1074/jbc.270.48.28499.
Recent evidence suggests that reactive oxygen species (ROS) may function as second messengers in intracellular signal transduction pathways. We explored the possibility that ROS were involved in lysophosphatidic acid (LPA)-induced mitogen-activated protein (MAP) kinase signaling pathway in HeLa cells. Antioxidant N-acetylcysteine inhibited the LPA-stimulated MAP kinase kinase activity. Direct exposure of HeLa cells to hydrogen peroxide resulted in a concentration- and time-dependent activation of MAP kinase kinase. Inhibition of catalase with aminotriazole enhanced the effect of LPA on induction of MAP kinase kinase. Further, LPA stimulated ROS production in HeLa cells. These findings suggest that ROS participate in the LPA-elicited MAP kinase signaling pathway.
最近的证据表明，活性氧（ROS）可能作为细胞内信号转导途径中的第二信使发挥作用。我们探讨了ROS参与溶血磷脂酸（LPA）诱导的HeLa细胞丝裂原活化蛋白（MAP）激酶信号通路的可能性。抗氧化剂N-乙酰半胱氨酸抑制了LPA刺激的MAP激酶激酶活性。将HeLa细胞直接暴露于过氧化氢会导致MAP激酶激酶的浓度和时间依赖性激活。用氨基三唑抑制过氧化氢酶增强了LPA对MAP激酶激酶诱导的作用。此外，LPA刺激HeLa细胞中ROS的产生。这些发现表明ROS参与了LPA引发的MAP激酶信号通路。
