Chernobseva I D, Kalinka R F, Dalmane A R
Arkh Anat Gistol Embriol. 1977 Dec;73(12):72-6.
An attempt has been made to reveal simultaneously both mast and ECL cells in the fundic mucosa of some laboratory animals and man. In Bouin fluid fixed specimens, toluidine blue pH 5.0 and alcian blue pH 1.0 failed to reveal mucosal mast cells in rats and mice only. In those animals mucosal mast cells became demonstrable in Carnoy fluid fixed tissues after staining with alcian blue pH 1.0. A double staining technique has been applied using Grimelius silver method followed by staining either with toluidine blue after acid hydrolysis or with alcian blue. Both mast and ECL cells became visible showing here and there their close arrangement. The latter might be a point for some functional relations between both cell types.
人们试图同时揭示一些实验动物和人类胃底黏膜中的肥大细胞和肠嗜铬样(ECL)细胞。在经布安液固定的标本中,pH 5.0的甲苯胺蓝和pH 1.0的阿尔辛蓝仅未能显示大鼠和小鼠的黏膜肥大细胞。在这些动物中,用pH 1.0的阿尔辛蓝染色后,在经卡诺伊液固定的组织中可显示出黏膜肥大细胞。采用了双重染色技术,先用格里梅利乌斯银染法,然后在酸水解后用甲苯胺蓝或用阿尔辛蓝染色。肥大细胞和ECL细胞均清晰可见,显示出它们在各处紧密排列。后者可能是这两种细胞类型之间存在某些功能关系的一个点。