Kawahara K, Fukunaga M, Takata T, Kawamura M, Morishita M, Iwamoto Y
Department of Preventive Dentistry, Hiroshima University School of Dentistry, Japan.
J Periodontol. 1995 Sep;66(9):775-9. doi: 10.1902/jop.1995.66.9.775.
The distribution and the density of gamma delta T cells in human gingival tissues were examined immunohistochemically in biopsy samples obtained from 20 subjects. Few gamma delta T cells were observed in gingival tissue free from inflammatory cell infiltration, but were found, albeit in low numbers, in association with inflammatory cell infiltration, especially T cells. This relationship with T cells was confirmed statistically. The ratios of gamma delta T cells to T cells in the epithelia and in the connective tissue were calculated in the sections in which more than 500 CD3-positive cells were identified. Seven of eight such epithelial specimens showed a ratio of less than 1% and one less than 2% (mean +/- SD; 0.8% +/- 0.4). In the connective tissue, 8 of 13 such specimens showed less than 1%, three less than 2%, one 3%, and one 7% (1.4% +/- 1.9). These results suggest that basically gamma delta T cells are not resident cells in the gingival epithelium such as comprise the first defense line against exogenous irritation. They may play some role in the pathogenesis of periodontal disease collaborating with alpha beta T cells in the inflammatory response.
通过免疫组织化学方法，对20名受试者的活检样本中人类牙龈组织内γδT细胞的分布和密度进行了检测。在无炎症细胞浸润的牙龈组织中，几乎未观察到γδT细胞，但在伴有炎症细胞浸润，尤其是T细胞浸润的组织中，虽数量较少，但能检测到γδT细胞。这种与T细胞的关系得到了统计学证实。在识别出500多个CD3阳性细胞的切片中，计算上皮组织和结缔组织中γδT细胞与T细胞的比例。在8个此类上皮标本中，有7个显示该比例低于1%，1个低于2%（平均值±标准差；0.8%±0.4）。在结缔组织中，13个此类标本中有8个显示低于1%，3个低于2%，1个为3%，1个为7%（1.4%±1.9）。这些结果表明，γδT细胞基本上不是牙龈上皮中的常驻细胞，而牙龈上皮是对外源性刺激的第一道防线。它们可能在牙周疾病的发病机制中发挥某种作用，在炎症反应中与αβT细胞协同作用。
